Dissertation > Excellent graduate degree dissertation topics show

Cloth and β- elemene combined administration of anti-tumor effect and mechanism of celecoxib

Author: WuZuo
Tutor: XuGuangLin
School: Nanjing Normal University
Course: Microbial and Biochemical Pharmacy
Keywords: Hepatocellular carcinoma cells Celecoxib Elemene Proliferation inhibition Apoptosis
CLC: R96
Type: Master's thesis
Year: 2011
Downloads: 8
Quote: 0
Read: Download Dissertation


ObjectiveMeasure the effect of Celecoxib &β-Elemene on proliferation inhibition and apoptosis induction of human hepatocellular carcinoma cell line HepG2, and reserch its possible mechanism. Detarmine the effect of combination therapy, provide a theoretical basis for further study.MethodsThe carcinoma cells HepG2 were treated with Celecoxib and elemene singly and in combination. Cell proliferation was determined using MTT assay; Apoptosis was detected using AO/EB douby staining with fluorescence microscope;cell apoptosis and the cell cycle was determined using FCM; Expression of apoptosis-releted proteins, COX-2, p38and P-p38 was determined using Western Blot. ResultsClecoxib or elemene alone could significantly inhibited HepG2 cell droliferation in a dose-dependent manner, and the inhibitory effect of Celecoxib combined with elemene was a joint synergy, The results of FCM showed the cell cycel was blocked in G2 phase. AO/EB doubly staining showed the cells appered typical characteristics of apoptosis. FCM showed that both of the two drugs induced HepG2 cells apoptosis. and has a synergistic effect. Results of Western Blot showed that the expression of Bcl-2, COX-2 was decreased; Bax, Caspase-3 expression increased; in the case of p38 expression unchanged, p38 phosphorylation levels rised. ConclusionBoth of Celecoxib and elemene can inhibit cell proliferation and promote cell apoptosis of human hepatocellular carcinoma cells HepG2,and combined use shows a synergic effect.

Related Dissertations

  1. The Study on Cryopreservation and Mechanism of Freezing Injury on the Spermatozoa of Coelomactra Antiquate,S968.3
  2. The Anti-tumor Effect of CADPE and Inducing Apoptosis in Human Gastric Cancer Cells,R735.2
  3. TRAIL in the regulation of tumor invasion CD4 ~ CD25 ~ Treg,R730.2
  4. The Study of Lead Exposure on Cyprinus Carpio Ovary Epithelial Cells,X174
  5. Effects of Diclazuril on G3PDH in Second-generation Merozoites of Eimeria Tenella,S858.31
  6. The Effects and Preliminary Study of PCV2 on Ca2+ Signal in Lymphocytes of Piglets,S858.28
  7. Effects of Angiogenic Factor on Follicular Angiogenesis and Development in Sexual Maturity Mice,S852.2
  8. Expression of β-Catenin in Pig’s Ovary and the Effect of β-Catenin on Porcine Granulosa Cells Apotosis and Steroidogenesis Related Enzyme,S828
  9. Induced Combination Apoptosis by FB1 and AFB1 in Vero Cell,S856.9
  10. The Apoptosis Mechanism Induced by Aflatoxin B1 and Deoxynivalenol in Primary Hepatocyte of Cyprinus Carpio,S856.9
  11. Effects of BMPR-IB Gene Silencing by Small Interfering RNA on Apoptosis of Porcine Pollicular Granulosa Cells and Ecpression of BMP Pathy-Way-Ralted Genes,S828
  12. The Effects of Don on Proliferation, Differentiation, and Apoptosis of Chondrocytes in Chicken,S858.31
  13. The Effect of Foxol on Apoptosis of Mouse Granule Cells,S865.13
  14. Study of Melittin on Tumor Inhibition and Part of Mechanism of Human Hepatocellular Carcinoma HepG-2 Xenograft in Nude Mice,R735.7
  15. The Protective Effect of Rat Myocardial Ischemia/reperfusion Injury Following Bone Marrow Mesenchymal Stem Cell Pretransplantation for 1 Week,R542.22
  16. Expression of WW Domain Containing Oxidoreductase Gene in Cholangiocarcinoma and Its Effect on the Biological Behavior of Cancer Cell Line QBC939,R735.8
  17. The Effects of (180F-FDG on the Apoptosis of Eca-109 Esophageal Cancer Cell Line,R735.1
  18. The Signal Mechanism Underlying DON Induced Apoptosis in BHK-21 Cells,S856.9
  19. The Expression and Mechanism of NF-κ B and IL-6 after 90% Portal Vein Ligation in Rats,R657.3
  20. The Study of Mitochondrial Signal Mechanism Induced Apoptosis by Don in Human Colon Cancer Cells,R735.35

CLC: > Medicine, health > Pharmacy > Pharmacology
© 2012 www.DissertationTopic.Net  Mobile