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The Potential Function of 53BP2 in Cell Cycle and Apoptosis Regulation and the Preliminary Study of HDIs-sensitivity Function by Radiosensitizer in Tumor Cells

Author: ZhuXiaoMei
Tutor: ZhangYingJiu；SongYi
School: Jilin University
Course: Biochemistry and Molecular Biology
Keywords: 53BP2 p53 Apoptosis HDIs PIKK inhibitors
CLC: R730.5
Type: Master's thesis
Year: 2007
Downloads: 57
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Abstract


This topic 53BP2 function in the regulation of cell cycle and apoptosis and radiation sensitizer for HDIs induced apoptosis of tumor cells were studied : ( 1 ) Construction of plasmid containing 53BP2 full-length cDNA for tumor cells in transient over expression and build stable cell lines can be induced interference cell lines ; build 53BP2 stability ; using flow cytometry , Western Blot , RT-PCR was used to detect the cell cycle , the corresponding protein and mRNA levels . Detected of 53BP2 on cell apoptosis overexpression and knock down two , observed of 53BP2 on cell status and cell molecules , to explore the mechanism of its function . ( 2 ) use the ATM and ATR inhibitor Wortmannin of the Caffeine small molecule radiation sensitizer as a tool for drug to inhibit the activation of the DNA damage sensor molecules ATM, ATR , monitoring tumor cells (Molt-4, HeLa) the HDIs class drugs (TSA SAHA) reactive to explore such as ATM, ATR -mediated DNA damage signaling network in HDIs induced functional significance in tumor cell apoptosis . The study results show that p53 plays an important role in the function of 53BP2 promote apoptosis , and possibly through the confrontation of the inhibition of apoptosis protein survivin , and further strengthen its pro-apoptotic function . In addition , the γ-ray -induced DNA damage response capable of cell cycle progression impact , damage repair cells as well as tumor therapy is of great significance . On the other hand , found that the PIKK inhibitors can significantly enhance the HDIs induced apoptosis of tumor cells can be used as a new combination method , an important reference value to the research and development of new cancer treatment programs .

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CLC: > Medicine, health > Oncology > General issues > Tumor Therapy
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