Dissertation > Excellent graduate degree dissertation topics show

Study Differerce of Antitumor Mechanism of Tian Li Ke and Zedoary Turmeric Oil on HepG-2 Cell

Author: WeiShuTang
Tutor: MaLi
School: Lanzhou University
Course: Internal Medicine
Keywords: Days Lectra Volatile oil Hepatoma HepG-2 Cell lines Apoptosis survivin NF-κB Telomerase
CLC: R735.7
Type: Master's thesis
Year: 2007
Downloads: 142
Quote: 1
Read: Download Dissertation

Abstract


Objective To study comparing traditional Chinese medicine days Lectra single drug Volatile oil and 5-Fu in vitro on human hepatoma cell line HepG-2 anti-tumor effect and mechanism of the similarities and differences. Methods vitro cell culture technology, light microscopy, fluorescence microscopy and transmission electron microscopy to observe the changes in the cell structure, MTT method was used to observe cell growth inhibition, apoptosis and cycle flow cytometry, Hoechst 33258 staining reagents staining drug treatment of hepatocellular carcinoma HepG -2 cell morphological changes, KGI TRAP-silver staining study drug treatment of liver cancer HepG-2 cells, telomerase activity expression and immunocytochemistry technology research drug treatment of liver cancer HepG-2 cells, survivin, NF- κB protein expression. MTT assay confirmed Volatile oil significantly inhibited hepatoma HepG-2 cells, and there is a significant concentration - time-dependent; days Lectra hepatocellular carcinoma HepG-2 cells did not show inhibition. Microscopy days Lectra human hepatoma cell cause cytoplasmic vacuolar degeneration of cells showed typical apoptotic morphological changes seen after the volatile oil of Curcuma: cell chromatin condensation, gathered at the nuclear edge to form a block or crescent-shaped ; reduced cell volume, nuclear pyknosis stain, cell membrane blebbing, formation of apoptotic bodies. Flow cytometry results show days Lectra, after the Curcuma oil and 5-Fu role of S-phase cells increased the proportion of days Lectra role strongest (P <0.01). The Curcuma oil group similar to the role of 5-Fu group, and can induce apoptosis days Lectra group, negative control group a significant difference (P <0.05); AV / PI staining, flow cytometry days Lectra after phosphatidylserine (phosphatidyl-serine, PS) in the cell membrane valgus, does not appear late apoptotic; Curcuma oil ,5-Fu role in cell apoptosis, and the the Curcuma oil can be a direct result of cell necrosis, with the negative control group, days Lectra group ,5-Fu group difference was significant (P <0.05). Immunohistochemistry shows the days Lectra group, the Curcuma oil group and 5-Fu group Survivin protein and the expression of NF-κBp65 were lowered (P <0.05). Days Lectra group down the most obvious Survivin protein (P <0.01); KGI TRAP-silver staining role of Ezhu oil cells 24 h after the Curcuma oil group, days Lectra group and 5-Fu group telomerase activity decreased days Lectra The most obvious group role with the the Curcuma oil group ,5-Fu group have significant difference (p <0.05). Conclusion days Lectra main inhibition of cell proliferation, separatism and the Curcuma oil ,5-Fu induce apoptosis. Days Lectra significantly change the cell cycle, almost blocking all cells in the S phase, the strongest effect of the change of the cell cycle. Days Lectra only induced cell early apoptosis; Survivin protein and NF-κB downregulation of telomerase activity is the main mechanism that significantly reduce its anti-tumor effect.

Related Dissertations

  1. The Study on Cryopreservation and Mechanism of Freezing Injury on the Spermatozoa of Coelomactra Antiquate,S968.3
  2. The Anti-tumor Effect of CADPE and Inducing Apoptosis in Human Gastric Cancer Cells,R735.2
  3. Cloth and β- elemene combined administration of anti-tumor effect and mechanism of celecoxib,R96
  4. Study on Extraction and Active Function of the Chemical Composition of Cacumen Platycladi,R284
  5. TRAIL in the regulation of tumor invasion CD4 ~ CD25 ~ Treg,R730.2
  6. Study on the Extraction of Volatile Oil from Hangzhou White Chrysanthemum Morifolium and Its Application in Cigarettes,TS264.3
  7. The Study of Lead Exposure on Cyprinus Carpio Ovary Epithelial Cells,X174
  8. Effects of Diclazuril on G3PDH in Second-generation Merozoites of Eimeria Tenella,S858.31
  9. The Effects of PCV2 on NF-κB Signal in Piglet’s Lymphocytes in Vitro,S858.28
  10. The Effects and Preliminary Study of PCV2 on Ca2+ Signal in Lymphocytes of Piglets,S858.28
  11. Effects of Angiogenic Factor on Follicular Angiogenesis and Development in Sexual Maturity Mice,S852.2
  12. Expression of β-Catenin in Pig’s Ovary and the Effect of β-Catenin on Porcine Granulosa Cells Apotosis and Steroidogenesis Related Enzyme,S828
  13. Induced Combination Apoptosis by FB1 and AFB1 in Vero Cell,S856.9
  14. The Apoptosis Mechanism Induced by Aflatoxin B1 and Deoxynivalenol in Primary Hepatocyte of Cyprinus Carpio,S856.9
  15. Effects of BMPR-IB Gene Silencing by Small Interfering RNA on Apoptosis of Porcine Pollicular Granulosa Cells and Ecpression of BMP Pathy-Way-Ralted Genes,S828
  16. The Effects of Don on Proliferation, Differentiation, and Apoptosis of Chondrocytes in Chicken,S858.31
  17. The Effect of Foxol on Apoptosis of Mouse Granule Cells,S865.13
  18. Study of Melittin on Tumor Inhibition and Part of Mechanism of Human Hepatocellular Carcinoma HepG-2 Xenograft in Nude Mice,R735.7
  19. Nuclear Dot Protein NDP52 Bind to Tumor Necrosis Factor Receptor Associated Factor 6 and Their Clinical Significance of Research,R363
  20. Effective of the Interaction between NDP52 and TRAF6 and TRAFs on NF-κB Signal Pathway,R363
  21. The Protective Effect of Rat Myocardial Ischemia/reperfusion Injury Following Bone Marrow Mesenchymal Stem Cell Pretransplantation for 1 Week,R542.22

CLC: > Medicine, health > Oncology > Gastrointestinal Cancer > Liver tumors
© 2012 www.DissertationTopic.Net  Mobile