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Purpose of hepatocellular carcinoma (hereinafter referred to as liver cancer) is one of the common malignant tumors, with high invasion bags, early metastasis, recurrence, clinical and pathological features of poor prognosis. Numerous studies have demonstrated that the invasion and metastasis of liver cancer is caused mainly due to its poor prognosis. HCC metastasis and recurrence of the complexity of a multi-step process, cancer cells to complete the transfer of the need to go through multiple links, with the participation of a variety of factors, including the expression of extracellular matrix degradation, tumor angiogenesis, and cell adhesion molecules The exceptions are several important sectors. This paper was measured by immunohistochemical methods the four biomarker closely associated with the occurrence of the tumor cells, invasion and metastasis, ie the the model matrix metalloproteinase (Membrane-type 1 matrix Metalloproteinase MT1-MMP), matrix metalloproteinase induction factor (Extracellular matrix metalloproteinase inducer CD147/EMMPRIN), the pan pipe endothelial growth factor (Vascular endothelial growth factor VEGF) and adhesion molecules (CD44 variance exon 6 CD44v6), aims to explore the four biological markers in hepatocellular carcinoma tissues expression and its clinical significance; analysis of EMMPRIN, MT1-MMP, CD44v6 and VEGF expression levels and liver cancer metastasis, tissue differentiation, and understand their role in the process of liver cancer invasion and metastasis; clear four markers mutual synergies between whether the relationship is in the process of invasion and metastasis of liver cancer; further explore MT1-MMP, whether of EMMPRIN, VEGF and CD44v6 as biological indicators predict liver cancer prognosis. The present study data on the surgical specimens of 40 patients with HCC, 10 cases of normal liver tissue, using immunohistochemical methods, detection EMMPRIN, MT1-MMP, CD44V6, VEGF expression in human liver cancer tissue and normal liver tissue and combined with clinical data to conduct a comprehensive analysis. Results 1) EMMPRIN, MT1-MMP, CD44v6 and VEGF in hepatocellular carcinoma and positive expression rate of 62.5%, 52.5%, 58.5% and 77.5%, respectively, in the normal liver tissue positive expression rates of 0-30% by the χ ~~ between 2 test, P value of <0.01, the difference was statistically significant; 2) EMMPRIN metastasis of hepatocellular carcinoma, poorly differentiated group, diameter ≥ 5cm in positive expression rate with no transfer of well-differentiated group tumor diameter <5cm of cases, significant differences (P <0.05); 3) of MT1-MMP of VEGF and tumor's metastasis, tumor size was being related, nothing to do with liver cancer tissue differentiation degree of; 4) Expression of CD44v6 and the tumor's metastasis, differentiation were positively correlated, regardless of the size of the tumor; 5) of EMMPRIN protein and MT1-MMP protein in hepatocellular expression there is a positive correlation spearman correlation coefficient is r = 0.654, (P <0.05); of MT1-expression of MMP protein and CD44v6 protein in liver cancer in the expression of positive correlation, spearman correlation coefficient of r = 0.701, P <0.05; CD44v6 protein and VEGF protein expression in HCC positive correlation spearman correlation coefficient of r = 0.506, P <0.01; MT1-MMP protein and VEGF protein expression in HCC positive correlation, the spearman a correlation coefficient of r = 0.566, P <0.01. Conclusion 1) of EMMPRIN, MT1-MMP, CD44v6 and VEGF positive expression rate in HCC tissues was significantly higher than that of normal tissue, likely to play an important role in tumor development; 2) EMMPRIN, MT1-MMP, CD44v6, VEGF in tumor the positive expression of metastasis group were higher than the non-transfer group, there were significant differences, suggesting that their expression and liver cancer invasion and metastasis, it is possible to predict cancer recurrence, metastasis reference index; 3) of EMMPRIN and MT1-MMP, MT1- MMP and CD44v6 of CD44v6 and VEGF, MT1-MMP and VEGF expression in HCC tissues significantly positive correlation may be in different aspects, different degrees of interaction and reinforce each other and jointly promote the malignant behavior of liver cancer .
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