Dissertation > Excellent graduate degree dissertation topics show

Differentiation Induced by Panaxydol in HepG2 and SMMC-7721 Hepatocarcinoma Cells

Author: SongLi
Tutor: YinMing
School: Shanghai Jiaotong University
Course: Pharmacology
Keywords: Ginseng epoxy propargyl alcohol Hepatocellular carcinoma Cell differentiation
CLC: R735.7
Type: Master's thesis
Year: 2007
Downloads: 176
Quote: 0
Read: Download Dissertation

Abstract


Objective: To investigate ginseng epoxy propargyl alcohol on the human hepatoma cell line HepG2 , SMMC -7721 growth inhibition and differentiation-inducing effect . Methods : ( 1 ) MTT assay , light microscopy , transmission electron microscopy observation of ginseng epoxy alkynol HepG2, SMMC -7721 human hepatoma cell proliferation , morphology and ultrastructure . (2 ) measured by ELISA cytoplasmic alpha-fetoprotein levels , bromocresol green colorimetric determination of albumin, the diazo reaction colorimetric determination of gamma - glutamyl GGT vitality , colorimetric determination of alkaline phosphatase activity changes . (3) flow cytometry of the cell cycle treated with ginseng epoxy alkynol change . (4) RT-PCR method detection different concentration ginseng epoxy alkynol the processing above hepatoma cell lines Id1, Id2, p21 gene expression levels change . ( 4 ) Western - blot assay medication before and after the change of p21, Rb protein expression levels . Results: ginseng epoxy alkynol a time-and dose -dependent manner inhibition of human hepatoma cell line HepG2 , SMMC -7721 cell proliferation ; 6μmol / L the role of ginseng epoxy alkynol 4 days , can be effective in changing a vicious form of liver cancer cells and ultrafine structure characteristics ; 4 8μmol / L ginseng the epoxy alkynol treatment , HepG2, SMMC-7721 cells AFP secretion and γ-GT activity was significantly lower albumin secretion and alkaline phosphatase activity increased . Cell cycle analysis showed that the increase in the number of cells in G0/G1 phase , S phase decreased , suggesting the ginseng epoxy alkynol arrest at the G1 phase of the cell cycle . The ginseng epoxy alkynol is weakened regulation factor IdmRNA expression of the cell cycle , the negative regulator of p21 and tumor suppressor genes Rb gene and protein levels were increased . Conclusion : Low doses of ginseng epoxy propargyl alcohol -induced liver cancer cells HepG2 , SMMC-7721 to the differentiation of normal cells , which may be related by upregulating the expression of the tumor suppressor gene p21 and Rb to cut cell differentiation inhibiting factor Id gene expression , and resistance cell cycle delay and achieve .

Related Dissertations

  1. Cloth and β- elemene combined administration of anti-tumor effect and mechanism of celecoxib,R96
  2. Clinical Significance of STAT3 and MMP-2 in Hepatocellular Carcinoma.,R735.7
  3. Assessment of Efficicy of Transarterial Chemoembolization by Gelfoam Particles Using DSCT Perfusion Imaging for Hepatocellular Carcinoma,R735.7
  4. Gelatin Sponge Particles Embolization of Hepatocellular Carcinoma after Regulatory T Cells and Its Significance,R735.7
  5. Quantiative Proteomics of Liver Immortalized Cell Line Chang Liver and Liver Cancer Cell Line HepG2,R735.7
  6. Expression and Functional Role of Proton-regulated Channels in Hepatic Carcinoma Cells,R735.7
  7. The Influence of Exogenous Hyaluronic Acid on Chondrogenic Differentiation of Rabbit Bone Marrow Mesenchymal Stem Cells,R329
  8. Qingdao region of hepatitis B virus the BCP gene mutation and hepatitis B virus associated with primary liver cancer,R512.62
  9. Clinical Significance of Hepatitis B Virus Core Gene PCR-RFLP Patterns on the Course of Hepatocellular Carcinoma and Liver Cirrhosis in China.,R735.7;R575.2
  10. Expression and Significance of NGF and Its Receptors in Primary Hepatocellular Carcinoma,R735.7
  11. Reversing Effect of Curcumin on Multi-drug Resistance of Human Hepatocellular Carcinoma Bel7402/5-Fu Cell Line,R285.5
  12. Study on the Association of the Precore/Core Region Variations of Hepatitis B Virus and Hepatocellular Carcinoma in Qidong High-Risk Area,R735.7
  13. The Significance of MiRNA-148a→DNA Methyltransferase 1→MiRNA-148a Promoter Methylation in Hepatocarcinogenesis,R735.7
  14. A Preliminary Investigation of the Expression of SK4 in Hepatocellular Carcinoma,R735.7
  15. Mechanism Study of Ubiquitin-Proteasome-Dependent Degradation of CDK2 Involved in the Treatment of Acute Myeloid Leukemia by All-trans Retinoid Acid,R733.71
  16. Study on Induced Differentiation of Human Pluripotent Stem Cells into Germ Cells,R329
  17. Hyaluronic Acid Gel Induction MDSC Differentiation for Chondroid Cells,R329
  18. The Effect of PGE2 on Expression of Survivin in Hepatocellular Carcinoma,R730.2
  19. IL12 Polymorphisms, HBV Infection and Risk of Hepatocellular Carcinoma in a High-risk,R735.7
  20. Effect of Curcumin on HIF-1α and VEGF Expression in Hepatocellular Carcinoma Hep1 Cells,R735.7
  21. Regulatory Mechanisms of Interleukin 8 Production Induced by Tumor Necrosis Factor-alpha in Human Hepatocellular Carcinoma Cells,R735.7

CLC: > Medicine, health > Oncology > Gastrointestinal Cancer > Liver tumors
© 2012 www.DissertationTopic.Net  Mobile