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Study on Interleukin-8 Gene Expression and Gene Polymorphisms in Henoch-Schonlein Purpura Nephritis of Children

Author: LiuBing
Tutor: HeWeiXun
School: Shanghai Jiaotong University
Course: Pediatrics
Keywords: Interleukin-8 Gene Expression Gene polymorphism Purpura nephritis Child
CLC: R726.9
Type: Master's thesis
Year: 2007
Downloads: 125
Quote: 0
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Abstract


Allergic purpura (HSP) in the main pathological changes of necrotizing blood inflammation systemic disease. Purpura nephritis (HSPN) is secondary to HSP kidney damage, is one of the children's secondary glomerular diseases. In recent years, studies have shown that HSPN vasculitis due in the participation of many cytokines, interleukin -8 (IL-8), the role of the more obvious it is a neutrophil chemokine involved in immune complex-mediated vasculitis pathological process, and the HSPN the clinical performance is closely related; IL-8 gene based on its 3 'end of the 2767 single nucleotide polymorphisms, while presented as AA genotype in the crowd, GG GA-type IL-8 gene polymorphisms in patients with HSPN susceptibility are closely related. HSPN children peripheral blood of this research to further clarify of HSPN with the relationship of IL-8, IL-8 expression and gene polymorphism analysis of its expression, gene polymorphism and HSPN relationship, which assessment of the impact and role of IL-8 in the pathogenesis of HSPN. Subjects and methods. HSPN in children with acute phase of 42 cases, the HSPN recovery period of follow-up of children with 28 cases in the control group of 20 patients from healthy children. The acute phase group 42 cases, 28 cases and 20 cases of children in the control group recovery group method using Real-Time PCR determination of CT values ??of IL-8, and calculated Delter delter CT value, quantitative calculation of mathematical formulas RNA expression levels. Sick group of children with 68 cases (including acute group and the convalescent group of children) and a control group of children with 20 cases using Pyrosequencing (Pyrosequencing) IL-8 gene polymorphism analysis. Results of the the mean quantitative results. Peripheral blood RNA expression of IL-8: 42 cases of the acute phase of the case group children 2 - (3.5079 ± 1.14947), 28 cases of recovery group for 2 - (5.9782 ± .81948), 20 cases in the control group, 2 - (7.3095 ± 0.67266). RNA expression levels of IL-8 in the peripheral blood of children with acute phase was significantly higher than the recovery group and the healthy control group, convalescent group with the acute phase of the case group peripheral the average quantitative level of RNA expression of IL-8 has significantly lower, but still higher than the control. After analysis of variance, pairwise comparisons of significant differences was statistically significant (P lt; 0.05). 2.68 cases of illness group (acute group and recovery group), homozygous 41 cases (60.3%), AA-nine cases (13.2%), GG 32 cases (47.1%), heterozygous (GA-type) 27 cases (39.7%), five cases of homozygotes in 20 healthy control group (25.0%), AA genotype 2 patients (10.0%), GG genotype 3 patients (15.0%), heterozygous (GA type) 15 patients ( 75.0%). The frequency distribution of homozygous diseased group was significantly higher after test, P lt; 0.05. Suggesting that IL-8 homozygous purpura nephritis in children more susceptible. Contrast the diseased group and the control group in the IL-8 gene homozygous (AA / GG) frequency of occurrence, after inspection and correction by Fisher's exact test, P value lt; 0.05. I.e. homozygous GG frequency higher than the frequency of occurrence of the AA, and showed a statistically significant difference. Tip of children with purpura nephritis, homozygous relative susceptibility, especially in the GG genotype than the latter. And sample distribution to meet the the Hardy-Weiberg balance. Conclusion IL-8 involved in the pathogenesis of purpura nephritis and pathological processes, and may be related to the clinical manifestations; IL-8 genotype homozygous (AA / GG), GG genotype, increased frequency will cause allergic purpura easier progress to purpura nephritis. IL-8 gene polymorphism play a significant role in the process of purpura nephritis, prompted the incidence of purpura nephritis is likely related to their genetic background.

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CLC: > Medicine, health > Pediatrics > Pediatric surgery > Pediatric urology science
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