Dissertation > Excellent graduate degree dissertation topics show

The Effect of Anti Sera Against FSH on Male Reproductive System

Author: XuHuiRu
Tutor: YaoBing
School: Nanjing Normal University
Course: Developmental Biology
Keywords: Anti - FSH autoantibodies Spermatogenic cells Hormone Apoptosis
CLC: R698.2
Type: Master's thesis
Year: 2007
Downloads: 60
Quote: 0
Read: Download Dissertation

Abstract


To clarify the infertile male patients in vivo existence of anti-FSH autoantibodies, we established a high specificity and sensitivity, and part of the infertile male patients and normal fertility, serum anti-FSH ELISA for the detection of autoantibodies anti-FSH autoantibodies the determination, found that anti-FSH autoantibodies, distribution asthenospermia patients higher frequency is particularly significant in azoospermia patients, anti-FSH autoantibodies in the part of the male infertility patients, and may induced male, less, the pathogenic factors another asthenospermia. For further study of anti-FSH autoantibody on the male reproductive system, we established a synthetic peptide anti-FSH antibody rat model, found that the number of spermatogenic cells in the rat model of seminiferous tubules and lumen were reduced sperm count, and epididymal sperm count and swollen sperm percentage were significantly reduced, and also found abnormal epididymal sperm count increase. Indicating that anti-FSH antibody indeed had an impact on the spermatogenic function of rat testis. On the basis of the above studies, we tested the model serum hormone levels and testicular cell apoptosis was observed, in order to explore the possible mechanisms of spermatogenesis reduce anti-FSH caused by autoantibodies. The experimental results show that the rat model of immune 77 d and 91 d, serum FSH levels were significantly lower when the immune 105 d, FSH levels rebounded. Rat model of each time period compared with the control group, serum Inhibin B levels significantly reduced, no significant changes in serum LH levels. PCR and TUNEL assay results found that the ratio of Bax and Bcl-2 mRNA levels within each time period model rat testis, caspase 3 mRNA levels and spermatogenic cell apoptosis index were significantly higher. Accordingly, we speculated that the anti-FSH autoantibodies by antigen-antibody immune complex formation and in vivo FSH may cause the peripheral blood FSH levels, which may in turn affect the secretory activity of the cells to activate Bax-caspase 3 signaling pathway (or other possible pathway of apoptosis), induces germ cell apoptosis, leading to spermatogenic dysfunction. The long-term presence of anti-FSH antibody in vivo may cause the formation of some sort of compensatory mechanisms in the body, thus spermatogenesis may be a certain degree of recovery.

Related Dissertations

  1. The Study on Cryopreservation and Mechanism of Freezing Injury on the Spermatozoa of Coelomactra Antiquate,S968.3
  2. The Anti-tumor Effect of CADPE and Inducing Apoptosis in Human Gastric Cancer Cells,R735.2
  3. TRAIL in the regulation of tumor invasion CD4 ~ CD25 ~ Treg,R730.2
  4. The Study of Lead Exposure on Cyprinus Carpio Ovary Epithelial Cells,X174
  5. Study on the Morphology, Physiology and QTLS Mapping of a Dwarf Mutant Ari1327 from Cotton (Gossypium Hirsutum L.),S562
  6. Effects of Diclazuril on G3PDH in Second-generation Merozoites of Eimeria Tenella,S858.31
  7. The Effects and Preliminary Study of PCV2 on Ca2+ Signal in Lymphocytes of Piglets,S858.28
  8. Expression of β-Catenin in Pig’s Ovary and the Effect of β-Catenin on Porcine Granulosa Cells Apotosis and Steroidogenesis Related Enzyme,S828
  9. Induced Combination Apoptosis by FB1 and AFB1 in Vero Cell,S856.9
  10. The Apoptosis Mechanism Induced by Aflatoxin B1 and Deoxynivalenol in Primary Hepatocyte of Cyprinus Carpio,S856.9
  11. Effects of BMPR-IB Gene Silencing by Small Interfering RNA on Apoptosis of Porcine Pollicular Granulosa Cells and Ecpression of BMP Pathy-Way-Ralted Genes,S828
  12. The Effects of Don on Proliferation, Differentiation, and Apoptosis of Chondrocytes in Chicken,S858.31
  13. The Study on the Effect of the Transport Stress and Anti-stress Treatment on the Physiological Function of Beef Cattle,S858.23
  14. The Effect of Foxol on Apoptosis of Mouse Granule Cells,S865.13
  15. The Protective Effect of Rat Myocardial Ischemia/reperfusion Injury Following Bone Marrow Mesenchymal Stem Cell Pretransplantation for 1 Week,R542.22
  16. Expression of WW Domain Containing Oxidoreductase Gene in Cholangiocarcinoma and Its Effect on the Biological Behavior of Cancer Cell Line QBC939,R735.8
  17. The Effects of (180F-FDG on the Apoptosis of Eca-109 Esophageal Cancer Cell Line,R735.1
  18. The Study of Mitochondrial Signal Mechanism Induced Apoptosis by Don in Human Colon Cancer Cells,R735.35
  19. The Impact of Histone Deacetylase Inhibitor Trichostatin a on Proliferation and Apoptosis of Porcine Granulosa Cells,S828
  20. Expression and Their Significance of Apoptosis Regulatory Factors Survivin、XIAP and c-Jun、NF-κB in Epithelial Neoplasm of Ovary,R737.31
  21. Therapeutic Effect and Mechanisms of Derived Mesenchymal Stem Cells in Intracerebral Hemorrhage Rat,R743.34

CLC: > Medicine, health > Surgery > Urology ( urinary and reproductive system diseases) > Men 's sexual dysfunction > Male infertility
© 2012 www.DissertationTopic.Net  Mobile