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Background and Purpose: At present in different tissues of mesenchymal stem cells (mesenchyme stem cells, MSCs) can actually be regarded as different tissues of adult stem cells (withadult stem cells, ASCs). It can proliferate in vitro culture conditions, while maintaining a highly undifferentiated state. It has multiple differentiation potential, under inducing conditions can be directed to differentiate into osteoblasts, chondroblasts, myoblasts, adipocytes, and endothelial cells. Under suitable conditions, mesenchymal stem cells can also differentiate into nerve cells. For nearly 10 years, how to get a lot of inter-mesenchymal stem cells for clinical use to become one of the hot spots of stem cell research. A large number of previous studies using bone marrow mesenchymal stem cells (bone marrow mesenchyme stem cells), induced in vitro to differentiate into nerve cells, or bone marrow mesenchymal stem cells transplanted into rat brain successfully promote the recovery of neurological function after stroke. However, due to limited sources of bone marrow MSCs as invasive access; presence of bone marrow-derived MSCs have pathogenic factors may and amplification and differentiation of the marked decline in growth in its number of cells with age, looking for a alternative to bone marrow MSCs, mesenchymal stem cells, but also in order to obtain a sufficient number of seed cells, people began to study isolated from human umbilical cord blood culture has a multi-differentiation potential of mesenchymal stem cells possibility and can compensate for its shortcomings method. Cord blood and bone marrow contains mesenchymal stem cells, human umbilical cord blood-derived mesenchymal stem cells (human umbilical cord blood mesenchyme stem cells, HUCBMSCs) may be more primitive, proliferation and differentiation capabilities, therefore they provide a source of cell therapy a new choice. The experiment through separation, on HUCBMSCs, culture and amplification. Then HUCBMSCs implanted spinal cord injury (spinal cord injury, SCI) rats the site of injury, implanted and not implanted HUCBMSCs animal group behavior and histological observations, to study the effect of treatment of spinal cord injury. Objective: To investigate the effect of umbilical cord blood mesenchymal stem cells used in the treatment of spinal cord injury nerve repair, to provide experimental evidence for the clinical application of umbilical cord blood mesenchymal stem cells to repair spinal cord injury. Method: 1. Full-term fetus's umbilical cord blood collected under sterile conditions, heparin, lymphocyte separation medium separation of cord blood mononuclear cells were cultured as a medium to Mesencult TM , purification, amplification to obtain MSCs: 2. 46 adult Wistar rats were \Were randomly divided into three groups injury group (SCI), sham transplant group (SCI NS), transplantation group (sCI NS HUCBMSCs), 15 the first two groups, the third group of 16 rats were injury days, 1w, 2w, 4w behavioral evaluation. Behavioral rated BBB (Basso - Beatie-Bresnehan, BBB) score. 1w, 4w line HE staining picture inflammatory reaction observed transplantation and experimental groups, 4w immunohistochemical GFAP, NSE test cell survival and differentiation. Results: derived from umbilical cord blood mononuclear cells (MNCs) planted in adherent cells, can produce the specific cultures agent after mainly osteoclast-like cells and mesenchymal-like cells; After the third passage, these cells can be purified and amplified, the performance of a mesenchymal-like cells. HUCBMSCs transplantation can promote spinal cord injury in rat hind limb motor function improvement, significant improvement can be seen as soon as 2w, 4w BBB score significantly improved. 2w and 4w transplant group with injury group and sham transplant group differences were statistically significant (P lt; 0.05). HUCBMSCs after transplantation, no significant immune rejection, 4w immunohistochemistry visible when a large number of transplanted cells survive and can be reflected to the glial cells. Conclusion: 1. HUCBMSCs grow well in the mesenchymal stem cell-specific medium. 2. Satisfactory result HUCBMSCs transplantation in the treatment of spinal cord injury. 3. HUCBMSCs able to survive after transplantation and generate new glial cells.
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