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Studies on MTB Specific Recombination Antigen in TB Serology Diagnosis

Author: ChengGuoPing
Tutor: ZhangRunLing；LiKeSheng
School: Lanzhou University
Course: Clinical Laboratory Science
Keywords: Recombinant protein Mtb8.4 Recombinant protein 38KD Recombinant protein MPT64 Recombinant protein MPT63 Square law ROC curve cut-off value MTB Tuberculosis
CLC: R446.6
Type: Master's thesis
Year: 2007
Downloads: 197
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Abstract


Objective To establish a standard of Mycobacterium tuberculosis (mycobacterium tuberculosis, MTB)-specific antigen recombinant protein Mtb8.4 38KD MPT64 and MPT63 enzyme-linked immunosorbent assay (enzyme-linked immunosorbent assay, ELISA) detection system. Establish the detection system detects the serum of patients with TB (tuberculosis, TB), to determine the sensitivity and specificity of the detection of MTB-specific antigen recombinant protein, in an attempt to improve the level of clinical laboratory diagnosis of tuberculosis. Methods square law and the ROC (Receiver Operating Characteristic) curve method to determine the the MTB specific antigen recombinant protein Mtb8.4, 38KD, MPT64 and MPT63 enzyme-linked immunosorbent assay antigen concentration and enzyme-labeled secondary antibody concentration to ROC positive judgment \The four antigen protein ELISA detection system using established clinical specimens collected to the ROC curve was used to determine the sensitivity and specificity values ??of the four kinds of antigen protein. Tuberculosis antibody of the same batch of serum samples. Reorganization of the four kinds of specific antigen combined group and compare the sensitivity and specificity values ??of sputum smear group, tuberculosis antibody group, and group differences were statistically analyzed. Results 1. MTB-specific antigen recombinant protein Mtb8.4 of 38KD, MPT64 and MPT63 ELISA detection system antigen concentration and enzyme-labeled secondary antibody concentrations were 1:32000 and 1:1000; 1:64000 1:1000:1:64000 and 1:1000 and 1:128 000 and 1:1000. 2. Mtb8.4, 38KD, MPT64 and MPT63 four kinds of specific antigens to detect their respective antibodies, the sensitivity and specificity of 47.60% and 93%, respectively, 59.60% and 92.30%, 68.00% and 92.30%, and 70.41% and 87.25% the combined group specific antigen detection sensitivity and specificity of 82.4% and 88.3%. The sensitivity of sputum smear group and tuberculosis detection of tuberculosis antibody group were 42.2% and 51.9%, the tuberculosis antibody group specificity was 81.8%. Mtb8.4 protein with a 38KD protein and MPT63 protein was positive linear MPT64 in protein and a 38KD protein showed a positive linear correlation between the four antigens Although there is a certain correlation, but can not be mutually alternative, complementary four antigens ideal method for the detection of tuberculosis detection. 3. Specific antigen combined group with the classic acid-fast staining (sputum smears group) and compared the tuberculosis antibody assay for clinical use (tuberculosis antibody group), and the detection of tuberculosis are statistically significant. The sensitivity and specificity of the combined group specific antigen detection of Mycobacterium tuberculosis superior to the clinical use of the tuberculosis antibody detection (the tuberculosis antibody group). The sensitivity was significantly better than the classic acid-fast staining (sputum smears group). 4. TB the patient course Mtb8.4 protein and 38KD protein, was a positive linear correlation. Leukocytes (granulocytes) and the 38KD protein, showed a positive linear correlation. Serum albumin 38KD protein negative linear relationship exists. Conclusion 1. The establishment MTB specificity protein Mtb8.4 of, 38KD, MPT64 and MPT63 standardized ELISA detection system. 2. The recombinant protein specific antigen square method and ROC curves antigen concentration and enzyme-labeled secondary antibody concentration is a key link in the establishment of a standardized ELISA detection system. 3. Although there is a certain correlation between the four antigens, but can not be mutually alternative, four specific antigen joint mutual complementary detection most satisfactory method of detecting tuberculosis. Specific antigen combined group compared with either the classic acid-fast staining (sputum smears group) or compared with current clinical use the tuberculosis antibody assay (the tuberculosis antibody group), the detection of tuberculosis showed statistically significant. The sensitivity and specificity of the combined group specific antigen detection of Mycobacterium tuberculosis superior to the clinical use of the tuberculosis antibody detection (the tuberculosis antibody group). The sensitivity was significantly better than the classic acid-fast staining (sputum smears group). 4. Mtb8.4 protein and 38KD protein antigen antibodies is corresponding increased with the duration of the extension, and may become a condition assessment and determine the efficacy of indicators. But needs further clinical validation.

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