Dissertation > Excellent graduate degree dissertation topics show

Shock Wave Promotes Differentiation of Human Mesenchymal Stem Cells into Osteoplasts

Author: WangBaoGang
Tutor: LiuYi
School: Jilin University
Course: Surgery
Keywords: Shock wave Human bone marrow mesenchymal stem cells Osteogenesis Differentiation
CLC: R329
Type: Master's thesis
Year: 2007
Downloads: 65
Quote: 0
Read: Download Dissertation

Abstract


Purpose : To observe the shock waves induced by in vitro culture conditions healthy adult bone marrow -derived mesenchymal stem cells (hMSCs) to the osteoblast differentiation characteristics , study the suitability shock wave intensity , and to explore its mechanism . Methods: 1 , in vitro healthy adult bone marrow mesenchymal stem cells carry out the separation , subculture , after the identification of immunohistochemical methods , respectively, using the different intensity of low-energy shock waves to induce and observe cellular changes and to detect and determine the most good shock waves induced strength . Identified bone marrow mesenchymal stem cells , using the best strength of the shock wave induced by RT-PCR, and at different times , the change of the observed gene expression of c-fos and c-jun . Experimental results : the shock wave induced bone marrow mesenchymal stem cells to the osteoblast differentiation optimal intensity 8.5kV, 120 , osteogenesis was significantly better than the control group ( P lt; 0.01 ) . The optimum strength shock wave , c-fos and c-jun gene expression was significantly increased compared with the control group , 45min peaked . Conclusion: low-energy shock waves under appropriate conditions can induce differentiation of bone marrow mesenchymal stem cells into osteoblasts increased gene expression of c -fos and c-jun .

Related Dissertations

  1. Exoression of Resistin During Porcine Preadipocyte Differentiation and Its Infiuencing Factors,R587.1
  2. The Yishenhuoxue methods for the treatment of kidney stones extracorporeal shock wave lithotripsy of renal injury after clinical studies,R277.5
  3. Professor Wang Qi identified the body - of Diseases - dialectical combination of academic thought and clinical experience and treatment of chronic insomnia clinical studies,R249.2
  4. miRNA-23a into the regulation of muscle differentiation in C2C12 cells,Q952.5
  5. miR - 23 in mouse skeletal muscle expression and the regulation of skeletal muscle regeneration research,Q78
  6. Evaluation of Land Use Eco-security in Fujian Province,X826
  7. Variations of Host-Specialized and Migratory Biotypes of Cotton Aphid on the Mating Behavior and Genes Sequences of Mitochondrion DNA and Symbiotic Bacteria,S433
  8. Genetic Characterization and Differentiation of Natural Populations of Wild Soybean from Middle and Lower Valleys of Huanghe and Changjiang as Well as Their Genetic Relationship with the Cultivated Soybean,S565.1
  9. The Molecular Systematics and Classification of Triticeae Polyploids with St Genome,S512.1
  10. Effects of Isoprocarb Exposure on Differentiation, Migration and Neurite Outgrowth of the Key Cell During Neural Development in Vitro,R329
  11. The Study of Rabbit Bone Marrow Mesenchymal Stem Cells Separation Culture and Directional Differentiation,R329
  12. University Press Digital Publishing and Countermeasures,G237.6
  13. Genetic Diversity, Population Sturucture and Gene Flow of Helicoverpa Armigera (H(?)bner) in China,S435.622.3
  14. Identification of Biological Characteristics with Human Keloid Fibroblast-like Mesenchymal Stem Cells,R622
  15. Study on the Physiological Differentiation and Genetic Diversity of Exserohilum Turcicum in Northeastern China,S435.131
  16. Optimization Study on BHF Marketing Strategy,F274
  17. The Comparative Study About Some Growth Indexes and Spike Differentiation of Wheat Under Greenhouse and Field,S512.1
  18. Psoriasis vulgaris in different stages and card Hou T lymphocyte subsets,R275.9
  19. Effects of Ginsenoside Rg1 on Cell Proliferation, Secretion Function and ERK、p38 Signal Molecular in Human Umbilical Cord Blood Mesenchymal Stem Cells under Optimized Culture Condition,R285.5
  20. Human Adipose Tissue Derived Stem Cells Differentiate into Epidermal Cell and Fibroblasts,R329
  21. Experiment on Repeated Bone Harvest Chamber in Vivo,R329

CLC: > Medicine, health > Basic Medical > Human morphology > Human histology
© 2012 www.DissertationTopic.Net  Mobile