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The Investigation of Nerve Fibers Communication between Gigantocellular Reticular Nucleus and Cervical Spinal Cord Neurons

Author: MaLi
Tutor: GaoXiaoQun；CuiYaPing
School: Zhengzhou University
Course: Human Anatomy and Embryology
Keywords: Reticular formation Reticular nucleus of the giant cell c-fos Germ agglutinin - horseradish peroxidase
CLC: R329
Type: Master's thesis
Year: 2007
Downloads: 33
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Abstract

The background and purpose of the brainstem reticular is an integrated center of the central nervous system from the center constantly issuing impulses to the cortex of the brain, spinal cord and cerebellum and other brain regions play a regulatory role. Traditional network structure is regarded as the central nervous system within an ancient structure, diffusion. Within In addition to the small number of clear boundaries nuclei, it seems that is not easy to subdivide other nuclear group: In addition to the regulation of visceral activities (such as breathing, circulation) in the function, do not see what an important role. But further research may prompt brainstem reticular There are many more important and complex functions. Later learned about its structure, not like the original so simple. With the application of fluorescence histochemistry and immune cells chemical methods, many specific neurotransmitter neurons in the reticular formation was found. But so far, the network structure, there are many issues yet to be resolved. The basis of the central nervous system function derived from the morphological structure. Reticular efferent fibers mainly projected onto the four regions: the hypothalamus, spinal cord, cerebellum and brainstem structure. Its role in excitatory and inhibitory regulation of skeletal muscle fibers in the spinal cord have an important role to regulate the random people limb movements and accuracy. In the network structure regulation and efferent fibers to the spinal cord, the spinal cord neurons projecting fibers to the reticular mesh structure is involved in the regulation of motor neuron feedback and control. Learn more about fiber connections between the reticular formation and spinal cord neurons, the network structure to provide a more theoretical basis for the regulation of movement, we select a network structure is closely associated with the movement-control extensor inhibition region important nuclei - giant reticular nucleus (gigantocellular reticular nucleus, NGC) to conduct a study to explore it and cervical spinal cord fiber links. Method 1, wheat germ agglutinin - horseradish peroxidase microinjection: select adult rats, microinjection of germ agglutinin - horseradish peroxidase neural tracer the rat right cytomegalovirus reticular nucleus. While establishing the negative control group for comparison. 2, wheat germ agglutinin - horseradish peroxidase detection: injection 48h with TMB substrate was detected cytomegalovirus reticular nucleus and cervical spinal cord gray germ agglutinin - horseradish peroxidase expression and data acquisition and analysis. 3, the trace of kainic acid injection: the same the rat right cytomegalovirus reticular nucleus microinjection of kainic acid, caused by noxious stimulation of the nerve fibers in the reticular nucleus of cytomegalovirus. The case of the detection of the c-fos gene expression: kainic acid injection testing laboratory rat cervical spinal cord gray matter within the c-fos gene expression after 2h, to conclude that the giant reticular nucleus and spinal cord fiber links. Results in the reticular nucleus of the giant cell injection germ agglutinin - horseradish peroxidase, can be found in the cervical spinal cord gray matter Ⅰ, Ⅱ lamellar anterograde mark in retrograde labeling Ⅶ, Ⅷ lamellar found. Giant reticular nucleus kainic acid injected noxious stimuli can cause cervical spinal cord gray VII Ⅷ lamellar c-fos gene expression has significant difference compared with the negative control group to α = 0.05 for the inspection level, the difference between the two groups is statistically significant. Conclusions direct fiber link between the mainly cytomegalovirus reticular nucleus of the brainstem reticular zone of inhibition and cervical spinal cord neurons, WGA-HRP tracing and c-fos gene expression detection . 2 gigantocellular reticular nucleus projection fibers to the spinal cord through the cervical spinal cord gray matter I II lamellar and more. Cervical spinal cord gray matter more to the giant reticular nucleus projection fibers originated in Ⅶ, Ⅷ lamina.

The Investigation of Nerve Fibers Communication between Gigantocellular Reticular Nucleus and Cervical Spinal Cord Neurons

Abstract

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