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Objective: through the extraction and separation of the traditional Chinese medicine compound \requirements further \three bend soup \Study of research methods: (a) content was determined by HPLC the active ingredient amygdalin bend soup Group Fangzhong bitter almond, ephedra, ephedrine hydrochloride and pseudoephedrine hydrochloride ephedrine. (B) amygdalin, ephedrine hydrochloride, pseudoephedrine hydrochloride, glycyrrhizin, glycyrrhizin content as an indicator, the use of orthogonal design method and the the uniform experimental method preferred \extraction and separation of the parts of the purification process. Results and Conclusions: The study shows that: (a) of ephedrine hydrochloride in the 0.043 ~ 0.366μg was a good linear relationship (r = 0.9999), repeatability experiment RSD of 0.78% and an average recovery rate of 101.03% (RSD1.53%), hydrochloric acid The pseudoephedrine in 0.021 ~ 0.182μg was a good linear relationship (r = 0.9994), repeated experiment RSD2.22% average recovery rate of 101.94% (RSD 1.88%), the method is simple, high sensitivity. The amygdalin at 0.260 ~ 1.236μg was a good linear relationship (r = 0.9998), repeated experiment RSD1.44%, with an average recovery rate of 95.79% (RSD 2.02%), the method is simple. (B) (1) ephedra effective parts best extraction and purification conditions: herbs with 8 times the water return extracted three times, each time 90min, water extract was concentrated under reduced pressure to 4 times amount of 40% NaOH solution adjusted to pH 11, 2 times, the combined ethyl acetate portion was extracted with ethyl acetate (1.5 times), and concentrated under reduced pressure to obtain ephedrine and pseudoephedrine crude extracts dried at 80 ° C under reduced pressure ie. (2) The bitter almond effective part of the best extraction and purification conditions: herbs with six times the 85% ethanol reflux extracted three times, each 60min, alcohol extract decompression concentrated to almost no alcohol taste, 3 times herbs weight of 95% ethanol ,2-4 ℃ soaking 36h. Filtration, the filtrate was concentrated bitter almond glycosides crude extracts dried at 80 ° C under reduced pressure that was. (3) licorice effective fraction was purified by optimal extraction conditions: herbs with 4 times of 0.3% aqueous ammonia, soaked for 3 times, each time 36h, combined leaching solution, adjusted with concentrated sulfuric acid pH6 ~ 7 and concentrated to 2 times amount will concentrate sample AB-8 macroporous resin adsorption chromatography column (the amount of resin 100ml column diameter 2.5cm, the column height 22cm), washed with water, 30% ethanol and 60% ethanol, 50ml bottle of eluate collected elution end point, to the detection of the thin layer chromatography, 60% ethanol eluate was collected and concentrated under reduced pressure to give the glycyrrhizin and glycyrrhizin extracts dried at 80 ° C under reduced pressure ie. Effective parts of this project to determine the impact the three bend soup of \not less than 50% of the effective parts to achieve the requirements set by the Chinese medicine five categories of new drugs, and has laid a good foundation for further \
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