|
Cassia genus (Sophora) plants belonging to the legume (Leguminosae), the whole genus of about 90 species, distributed in temperate and subtropical regions, about 30 kinds of north and south are produced. The chemical composition has isolated and identified from the genus alkaloids, flavonoids, phenols, terpenes, and other types of compounds. Cassia genus Sophora (Sophora flavescens Aition) a National outer most parts of Qinghai, Xinjiang produced. Chinese medicine Sophora bitter, cold, heat and dampness, insecticide, diuretic effect. The main active ingredient for the of the matrine, oxymatrine Sophoridine sophocarpine alkaloids and flavonoids isopentenyl branched of two categories. To further tap the active ingredients of traditional Chinese medicine, the paper dried roots of traditional Chinese medicine Sophora study of the chemical composition of the system. By column chromatography (CC), preparative thin layer chromatography (PTLC to), recrystallization was isolated over 60 compounds, and the use of UV, IR, MS, 1 H NMR 13 C NMR, DEPT, 2D-NMR, NOE, CD and optical rotation modern spectroscopic techniques, combined with literature data and standard control structure of the 58 compounds were identified. Mainly isopentenyl flavonoids, pterocarpan, isoflavones, phenylpropanoid, lignans and triterpenoids. 9 new compounds, including two flavonoids dimer, the two Dihydroflavonol two Dihydroflavonol, a flavonol, a pterocarpan derivatives and lignans. In addition, there is a cis-chalcones as for the first time isolated from nature obtained. In order to verify the flavonoids biosynthetic pathway of the dimer and to meet the needs of the biological activity test, the use of asymmetric synthesis methods, by chiral catalyst compound 24 (Flavonoids dimer) were semi-synthetic. In order to find the active ingredient of the plant species, on the part of the compounds obtained were the antimicrobial activity of the test, and found that the compound 3,10,14,21 against Staphylococcus aureus compound 10,14,21,36 of Bacillus subtilis, and the compound 1,10,14,36 good inhibition against E. coli. Prenylated flavonoids have special biological activity, the hot research since the 1970s, the structure and spectral characteristics of Sophora Prenylated flavonoids reviewed. This thesis has rich natural product database, is expected to make a greater contribution to plant chemotaxonomy, Drug Screening, and biosynthesis.
|