Dissertation > Excellent graduate degree dissertation topics show

Study on the Soil Environmental Security of SrMV-P₁ Gene Transgenic Sugarcane

Author: FuYunXia
Tutor: ZhangMuQing
School: Fujian Agriculture and Forestry University
Course: Crop Cultivation and Farming System
Keywords: Cane SrMV-P1 Transgenic Soil environment Security
CLC: S566.1
Type: Master's thesis
Year: 2008
Downloads: 25
Quote: 0
Read: Download Dissertation

Abstract

In this study, in turn SrMV-P ₁ gene sugarcane as material, through the Soil DNA and grass weeds (goosegrass) DNA PCR detection research source SrMV-P _{< / sub> horizontal gene transfer; transgenic sugarcane on soil microbial microbial plate culture studies; analysis of transgenic sugarcane on soil physical and chemical properties of the soil enzyme activity and nutrient changes and turn in a preliminary study SrMV-P ₁ Gene sugarcane soil environmental security. The results show that: in the the sugarcane rhizosphere microbial genomic DNA and grass weeds (goosegrass) genomic DNA was not detected exogenous SrMV-P ₁ genes, indicating SrMV-P ₁ gene horizontal transfer did not occur. Sugarcane residue decomposition, soil microbial quantity on the turn was no significant difference between the gene sugarcane and non-genetically modified sugar cane; sugarcane rhizosphere soil microbes in the mid potted plants inoculated sugarcane mosaic virus in sugarcane growth was significantly higher than that of unvaccinated; potted sugarcane the rhizosphere soil bacteria and actinomyces early in the growth of sugar cane, significantly higher than the growth of the late, rhizosphere soil fungi number sugarcane highest tillering stage; rhizosphere soil field cultivation, the number of bacteria in sugarcane growth period no significant difference in the actinomycetes in the tillering stage up to the highest, fungi maturity reached the highest level; transgenic sugarcane rhizosphere soil put actinomycetes and fungi is more complex, but have little impact on rhizosphere bacteria. The rhizosphere soil enzyme research results show that: the transgenic sugarcane residue decomposition of alkaline phosphatase and catalase significantly but other enzymes; different cultivation methods, potted unvaccinated sugarcane higher than the potted plants inoculated rhizosphere soil invertase, urease and catalase activity, alkaline phosphatase activity is lower than potted plants inoculated potted unvaccinated sugarcane rhizosphere soil acid phosphatase activity with alkaline phosphatase contrary; different growth period, rhizosphere soil invertase and urease activity late in the growth of sugar cane higher, while the two phosphatase and catalase activity reduce with the growth of the sugar cane; transgenic sugarcane rhizosphere soil urease and acid phosphatase activity has little effect, while both a certain effect on the activity of sucrase, alkaline phosphatase, and catalase. More potted plants inoculated under different cultivation methods, rhizosphere soil pH showed a weak acid, potted unvaccinated sugarcane rhizosphere soil pH is high; different growth period, rhizosphere soil pH in the pot along with the growth of sugar cane first down then up, but slightly increased with the growth of sugar cane is planted in the field; sugarcane growth in the late transgenic sugarcane slightly to reduce soil pH, potted plants genetically modified sugar cane residues also reduce soil pH. Ota between 0.108 ~ 1.24ms/cm potted sugarcane rhizosphere soil conductivity between 1.08 ~ 3.08ms/cm; With the growth of sugar cane rhizosphere soil conductivity first increased and then decreased slightly; sugar cane residues soil conductivity to 0.18 ~ 0.26ms/cm; and the transgenic sugarcane debris rhizosphere soil conductivity is more complex. The rhizosphere soil nutrient analysis showed that: 1) transgenic sugarcane residue decomposition of most soil nutrients (total nitrogen except) had no significant effect; 2) different cultivation methods, potted unvaccinated sugarcane rhizosphere soil total nitrogen, quick phosphorus and organic matter content and potted plants inoculated considerable; rhizosphere soil phosphorus and potassium content than potted plants inoculated; rhizosphere soil potassium, available nitrogen content is lower than the potted plants inoculated; 3) different growth period, root The rhizosphere soil total nitrogen and total phosphorus, phosphorus and potassium, and organic matter content are reduced with the growth of sugar cane; while rebounded somewhat after the first drop of the potassium content of the soil with the growth of sugar cane rhizosphere; rhizosphere soil available N The content of elongation highest; 4) transgenic sugarcane on soil available nutrients and organic matter content is more complex, but has little effect on total nitrogen, total phosphorus, and potassium. Preliminary studies indicate that the transgenic sugarcane rhizosphere soil enzymes, pH and conductivity are given a certain impact, the impact on soil available nutrients and organic matter content is more complex, but the impact of the total nitrogen, total phosphorus and total potassium small; different growth stages of different cultivation methods or sugar cane are likely to cause a disparate impact on soil physical and chemical properties.}

Study on the Soil Environmental Security of SrMV-P1 Gene Transgenic Sugarcane

Abstract

Related Dissertations

Study on the Soil Environmental Security of SrMV-P₁ Gene Transgenic Sugarcane