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Background: Coronary heart disease is a high incidence and serious health hazard for cardiovascular disease. Angiogenesis therapy therapeutic angiogenesis in ischemic diseases such as coronary heart disease patients with a wide range of market potential. Variety of vascular growth factor gene therapy to promote angiogenesis role has been recognized, but there are still issues such as efficacy and safety in clinical applications. In recent years, Chinese medicine therapeutic angiogenesis research made a lot of achievements. Studies have confirmed kidney Chinese medicine has a role in promoting angiogenesis. Morinda (Radix morinda officinalis, RMO) is commonly used in traditional Chinese medicine of Invigorant. Morinda oligosaccharides (Morinda officinalis How oligosaccharides, MOO) is the principal active ingredient in the Morinda day alcohol extract water-soluble part. The mentor group, previous studies have shown that, MOO has significant anti-hypoxia reoxygenation injury protection myocardial effects. However, whether it has a role in promoting angiogenesis, myocardial protective effect whether its to promote the ischemic myocardium collateral circulation, has not been reported. The subject mentor group on the basis of previous studies, aimed at preparation chick chorioallantoic sac membrane (chick chorioallantoic membrane, CAM) model of acute myocardial infarction (acute myocardial infarction, AMI) model, observe the MOO CAM angiogenesis in ischemic myocardium after AMI in rats. Morinda protective effect on cardiovascular elaborated from the perspective of therapeutic angiogenesis explore the mechanism and lay the foundation for further in-depth study and discussion of Morinda role in cardiovascular. Method: 1. MOO preparation of serum containing 16 Wistar rats were randomly divided into the MOO small, in the high-dose group and blank serum group, n = 4. 4d gavage method, each d, respectively 0.7g/kg, 1.4g/kg, 2.8g/kg ig Morinda water-soluble portion of the ethanol extract and the volume of saline (normal saline, NS), the last irrigation the stomach after 1h, abdominal aortic blood were prepared MOO small, medium, large doses containing serum and blank serum. 2. MOO promote angiogenesis in CAM experiments 60 chick embryos were randomly divided into NS group, blank serum, basic fibroblast growth factor (basic fibroblast growth factor, bFGF) group and small MOO, in the high-dose group, n = 10 only. Establish CAM model after incubation 7d, NS, blank serum, bFGF (2500U/ml), MOO small, medium, large doses of the drug-containing serum were added on the surface of the carrier in the CAM, continue incubation 3d after the preparation of CAM specimens stereomicroscope under the observation of the performance of angiogenesis and twelve vessel count. 3. MOO promote AMI angiogenesis in ischemic myocardium experiment 40 male Wistar rats, ligation of the left anterior descending artery, made a model of acute myocardial infarction (AMI) were randomly divided into model group Shexiangbaoxin, pill group, MOO small, medium, and high-dose group, n = 8. In another 10 set to false ligation group. Pill group Shexiangbaoxin modeling d the 30mg/kg Shexiangbaoxin pill suspension gavage; MOO modeling in the high-dose group d 0.7g/kg, 1.4g/kg, 2.8 g / kg orally Morinda water-soluble portion of the ethanol extract; equal volume of saline gavage d treatment group in the model group. False after ligation group without any treatment, conventional breeding. 24h after each treatment group in modeling drug intervention. 6w killed after myocardial drawn, paraffin-embedded sections, line general tissue morphology observed, detect microvascular density (MVD) in rats with ischemic myocardium, and vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF) protein expression, growth factor protein optical density measured using pathological image analysis system, and a semi-quantitative analysis. Results: 1. Blank serum group the angiogenesis performance nonspecific or specific radial extent of low the CAM angiogenesis performance NS group. bFGF group and MOO main vessel containing serum group closer to the carrier material, obvious proliferation of small and medium-sized blood vessels, especially in the small blood vessels to the carrier centered radially growth. 2. MOO containing serum on the CAM one, two angiogenesis blank serum, MOO, the high-dose group, two significant increase in the number of blood vessels (P <0.05), but were lower than pharmacophore bFGF group (P <0.05); compared with the MOO a small dose group, in the high-dose group, two the number of blood vessels was significantly increased (P <0.05), but no significant difference between the two. NS group and blank serum, two between the number of blood vessels without significant difference (P> 0.05). 3. Endomyocardial biopsy generally histomorphological observation false ligation group, model group the, Shexiangbaoxin pill group, MOO, in the high-dose group can see the newborn capillaries. Sham ligation group visible capillary proliferative obvious; model group, a small amount of capillary proliferation; MOO, middle dose group showed more hyperplasia of capillaries; Shexiangbaoxin pill group, and MOO large dose group, the majority of the proliferation of capillaries. 4. MOO ischemic myocardium after AMI MVD model rats compared with sham ligation group MVD was significantly increased (P <0.05). Compared with the model group, MOO, the high-dose group was significantly increased MVD (P <0.05), but the effect is weaker than Shexiangbaoxin pill group (P> 0.05);, although the MOO a small dose group MVD than the model group, but no significant difference (P> 0.05) between the two. MVD between the the MOO3 dose group showed a significant difference (P <0.05). 5. The MOO ischemic myocardium after AMI, VEGF, bFGF protein expression compared with sham ligation group, model group, Shexiangbaoxin pill group, MOO small in the high-dose group rat ischemic myocardium VEGF, bFGF optical density significantly increased (P <0.05); Shexiangbaoxin pill group increased most significantly, there were significant differences (P <0.05), and other groups. Compared with model group, MOO, the high-dose group a significant increase of VEGF, the of bFGF optical density values ??(P <0.05), but the role are weaker than Shexiangbaoxin pill group (P> 0.05); the MOO small dose groups of VEGF, bFGF The optical density values ??with model group, no significant difference (P> 0.05). MOO, the high-dose group the bFGF optical density value and the low-dose group had a significant difference (P <0.05), but in the middle, between the high-dose group had no significant difference (P> 0.05). VEGF optical density value between MOO3 dose group showed a significant difference. Conclusion: 1. Morinda the oligosaccharides can significantly promote angiogenesis of chick chorioallantoic membrane. 2. Morinda oligosaccharides after AMI significantly promote angiogenesis in rat ischemic myocardium, improve ischemic myocardial collateral circulation. 3. Morinda officinalis oligosaccharides can significantly promote the ischemic myocardium after AMI, VEGF, bFGF protein expression, which may be one of the pro-angiogenic mechanism.
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