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Guizhou is rich in folk medicine resources, such as Dong Miao medicine and drugs. To further tap the potential of Guizhou local ethnic medicinal value , we take 86 kinds of people in Guizhou local herbs as samples , the establishment of a sample library . And Candida albicans , Aeromonas Porphyromonas gingivalis and Streptococcus mutans , respectively, as fungi, Gram-negative and Gram -positive bacteria samples representative of the 86 kinds of samples stored in the repository for high antibacterial activity throughput screening , obtained with samples of 19 kinds of antibacterial activity , which has no antibacterial activity of two plants at home and abroad reported . By solvent extraction methods have been ringing grass, stone lotus seeds , lily, Usnea four species in water, 95% ethanol and ethyl acetate extracts , and Staphylococcus aureus , Escherichia coli, Enterococcus faecalis , Pseudomonas aeruginosa, Micrococcus luteus and Aspergillus as test bacteria, examine the various extracts of microorganisms for the six minimum inhibitory concentration and zone of inhibition size. In these experiments based on the results by column chromatography gradient elution of ethyl acetate extracts grass rings , stone lotus seeds 50% ethanol extracts of different components , and Staphylococcus aureus and Escherichia coli tested strains , grass and stone lotus seeds detected ringing each component of these two bacterial minimum inhibitory concentration and inhibition zone , in order to further isolate the compound has a good foundation for antibacterial activity , but also for these types of further development and utilization of plant and lay a good theoretical basis.
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