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Study on Quality Evaluation of Radix Lindera Reflexa

Author: ShenGuiFen
Tutor: XiongWeiZheng
School: Henan College of Traditional Chinese Medicine
Course: Of Pharmacy
Keywords: Lindera reflexa Hemsl quality standard effect factor fingerprint
CLC: R282
Type: Master's thesis
Year: 2007
Downloads: 171
Quote: 0
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Abstract


Objective:Through the systematic research on Radix Lindera Reflexa (RLR), to establish the determining methods of main chemical component content, volatile oil total content and extract of RLR and to perfect and elevate the quality standard. To formulate the optimal desirability of RLR, that is about the crude drug collection, processing specification, the storage modality and etc. These results can provide scientific basis for crude drug quality control and main ecological condition, could afford credible foundation for wildness changing to standardization growth and quality standard elevation. To inquire into HPLC determination from different habitats, to preliminary constitute the HPLC fingerprint of RLR. These consequences offer favorable basis and condition for quality standard further research and preparation craftwork innovation.Methods: By the systematic investigation on main chemical component content and thin-layer chromatography, volatile oil total content and extract of RLR from 10 different batches, for constituting the quality draft standard of RLR. Influences of quality factors on different parts, growth conditions and years, plucking ways and storage condition were investigated. Optimal qualification on collection, and processing specification, storage modality were formulated, for offering scientific basis on medicinal materials manufacturing, and ensuring the quality of RLR. Thirteen different chromatograms of RLR were collected. Studies on similarity evaluation and clustering analysis of chromatographic for RLR, and establish the HPLC fingerprint and quality evaluation methods of RLR.Results: Through the systematic study separation was performed on an Agilent HC-C18 column (150mm×4.6mm,5μm), with mobile phase nonlinear of MeOH-H2O (30%～100%) and with gradient elution at the flow rate of 1.0 ml/min. The pre-processing method was with methanol ultrasonic for one hour. The quality draft standards were those 3,5-didroxystilbene not less than 4.8mg/g, pinocembrin, 5,7-dihydroxy-flavanone not less than 0.8mg/g, pinostrobin,5,7-hydroxy-methoxy flavanone not less than 4.5 mg/g, volatile oil total content not less than 0.8%(ml/g),water soluble extract not less than 4.8% and alcohol soluble extract not less than 8.7%.The crude drug should be over three years, growing with a sunny exposure, gathering in winter or early spring and storing with whole or bulk. These were the optimal conditions of growing, storing and harvesting time. Thirteen batches of RLR from various producing areas were comparatively analyzed for methodology inspection. Thirteen batches were fractionated into three classes by similarity evaluation and clustering analysis of chromatographic for RLR. Eleven mutual peaks were selected in chromatography and a fingerprint was established.Conclusions: Our systematic research gains several results, the main chemical compositions’content has been determined and the draft standard of quality control has been formulated. The standards which are the optimal conditions of growing, storing and harvesting time have been instituted. These results could offer scientific basis on medicinal materials manufacturing and ensuring the quality of RLR. These results could provide scientific basis for crude drug quality control and main ecological condition, can afford credible foundation for wildness changing to standardization growth and quality standard elevation. The established fingerprint could be used for the quality control and species identifying of RLR. The accuracy, repeatability and stability of this method were satisfied. These consequences offer favorable basis and condition for quality standard further research and preparation craftwork innovation about RLR.

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CLC: > Medicine, health > Chinese Medicine > Of Pharmacy > Chinese herbal medicines
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