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Purpose observe the Yiqihuoxue side experimental AMI rats after myocardial tissue type Ⅰ, Ⅲ collagen, explore whether they have a role in anti-LVR, and explain its mechanism to provide experimental basis for clinical application and further development studies lay the foundation. Methods In this study, ligation of the left main coronary artery of male Wistar rats, the establishment of animal models of AMI were randomly divided into five groups, namely sham operation group, model group, Yiqihuoxue high-dose group (hereinafter referred to as the high-dose group), Qi blood circulation low-dose group (referred to as the low-dose group) medicine and captopril in the control group (control group), drug intervention 6 weeks, the rats of the general condition and left ventricular mass index (LVMI); using immunohistochemistry and RT-PCR analysis of myocardial tissue I and type III collagen expression. Results (1) rat general condition comparison Yiqihuoxue of the high-dose group and the control group can be significantly reduced in rats mortality after myocardial infarction in rat diet, activity in order to improve the general condition; compared with the model group were significantly different (P lt; 0.05); low-dose group the trend of reduction in mortality in rats, but not statistically significant compared with the model group (P GT; 0.05) LVMI values ??(2) rats LVMI comparison Yiqihuoxue party intervention after 6 weeks compared with model group for small, have a statistically significant difference (P lt; 0.05); (3) myocardial tissue of rats I and type III collagen expression comparison immune SP method: model group myocardial interstitial Ⅰ, type III collagen large number of hyperplasia, compare significant differences in sex (P lt; 0.05) with the sham group; high-dose group, the control group myocardial Ⅰ, type III collagen significantly reduce , compared with the model group were significantly different (P lt; 0.05); no significant difference between the high-dose group and the control group (P gt; 0.05); collagen content of the low-dose group in between the model group and the high-dose group, with both There was no significant difference (P gt; 0.05). RT-PCR test results showed that I and type III collagen mRNA in the basic synchronization expression at the protein level. Conclusion ① myocardial after AMI showed fibrosis, and the expression of type Ⅰ, Ⅲ collagen in the myocardium there is a close relationship. ② Yiqihuoxue square high-dose group and the control group after AMI rats have significant therapeutic effect, can significantly improve symptoms, improve the survival rate of mice. ③ Yiqihuoxue side by inhibiting post-MI rat cardiac I, type III collagen mRNA expression and reversal of cardiac fibrosis, the role of anti-LVR.
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