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The Interaction and Effect of Inoculation of AM Fungi, Phosphate-Solubilizing Bacteria and Rhizobium on Growth of Astragalus Sinicus L. at Different Phosphate Level

Author: XinWenJuan
Tutor: ZhaoBin
School: Huazhong Agricultural University
Course: Microbiology
Keywords: AM fungi Solubilizing bacteria Rhizobium Phosphorus levels Infection rate Alkaline phosphatase Succinate dehydrogenase nested-PCR
CLC: S154.3
Type: Master's thesis
Year: 2009
Downloads: 190
Quote: 0
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Abstract


Phosphorus is one of the three nutrient elements for plant growth, and only soluble phosphorus can be absorbed by plants to take advantage of. Because phosphorus can easily be fixed under natural conditions, which greatly limits the normal metabolism and growth of crops. Arbuscular mycorrhizal (AM) fungi are widely distributed in a class of beneficial microorganisms in the soil ecosystem, with more than 80% of terrestrial plants form mutualistic symbionts. AM fungi play an important role in promote host plant mineral nutrition absorption (especially phosphorus), and enhance the disease resistance of plants to environmental stresses. The rhizosphere soil insoluble phosphate solubilizing bacteria can be activated in order to improve the phosphorus content in the soil, rhizobia can fix atmospheric nitrogen, mycorrhizae to expand the range of root absorption of various nutrients mixed inoculation plants more The phosphorus yield effect to play a microbial Festival. Nature, AM mycorrhizal fungi diversity, and can not be a pure culture, morphological characteristics alone can not be identified to species, and when mixed AM fungal inoculation of two or more can not be understanding from the morphological characteristics of each fungal invasion dyed situation, this requires the help of molecular biology techniques. In this thesis, several strains of solubilizing bacteria dissolved Ca3 (PO4) 2 (TCP) and rock phosphate (RP) ability to select superior strains PA10 as potted experimental strains; milk vetch as host plants to study the different AM fungi phosphorus levels, Rhizobium, solubilizing bacteria affect milk vetch growth and the three fungi mutual relations. The papers results achieved mainly as follows: 7 strains were cultured in the liquid medium of the TCP inorganic phosphorus, all strains of the pH at 3.88-5.18. B (Bacillus megaterium) bacteria pH as low as 3.88 E (P. aeruginosa) the maximum amount of dissolved phosphorus for 288.30mg / L. HPLC detection of six kinds of organic acids, and the results show that the strain of phosphate solubilizing capacity and organic acid species closely related. Citric acid only in PA10 and PB5 detected, which may be dissolved and their RP relevant strongest. Solubilizing bacteria in the culture medium RP inorganic phosphorus, dissolved phosphorus less than the dissolved content of TCP phosphorus, PA10 dissolved phosphorus for 126.74mg / and. PA10 growth trend and the amount of dissolved phosphorus, pH some correlation, in which the amount of dissolved phosphorus and pH have a significant negative correlation (R2 = 0.957). Visible strains secrete organic acids. Reduce the pH of the solution is the main phosphate solubilization. On the other hand, in the pot experiment, mixed inoculation with AM fungi and phosphate-solubilizing bacteria, Rhizobium significantly promote plant growth is best strains combination three some synergies. The rhizobia activity close relationship with AM fungi. High phosphorus concentrations significantly inhibited the AM fungal infection rate, SDH and ALP activity. Pre-arbuscular abundance of AM fungi increased the activity of two enzymes also increased plant growth; disintegration of the large number of late plant growth arbuscular structure to collapse, and decreased activity. The results show that high P concentrations significantly inhibited the frequency of occurrence of the three mycorrhiza detected by nested-PCR fresh root segment three fungi relationship. Promoted in the 10 mg / kg of P in soil Glomus mosseae Glomus intraradices infection with the of Glomus geosporum on milk vetch. More stable with increasing phosphorus concentration, vitality and efficiency of G. Glomus mosseae was with G. intraradices, G. geosporum phosphorus concentration, infection and other sensitive microbial activity. In addition, the fresh root segments for nested-PCR detection, not only makes it easier to extract AM fungal DNA, sensitive and accurate, but also able to successfully detect the relationship between AM fungi.

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CLC: > Agricultural Sciences > Agriculture as the foundation of science > Soil > Soil Biology > Soil microbiology
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