Dissertation > Excellent graduate degree dissertation topics show

Effects of Parameters on Bacterial Community Structure in Agricultural Waste Composting

Author: ZhangJiaChao
Tutor: ZengGuangMing
School: Hunan University
Course: Environmental Science and Engineering
Keywords: composting bacterial community DGGE (denaturing gradient gel electrophoresis) redundancy analysis (RDA) multivariate analysis
CLC: S141.4
Type: Master's thesis
Year: 2009
Downloads: 204
Quote: 0
Read: Download Dissertation

Abstract


Only 0.1% to 10% of the microbes from natural habitats can be cultured, so it can’t well reflect the microbial diversity in the natural environment of the original state. With the development of molecular biology and phylogenetic analysis, based on 16S rDNA gene molecular biology methods such as polymerase chain reaction- denaturing gradient gel electrophoresis （PCR-DGGE） are gradually being widely used to analyze micrbial communities of different complex ecosystems. In this paper, the total DNA was amplified with PCR using the GC-338F/518R primers to obtain the bacterial 16S rDNA genes. The amplified products were separated and identified by DGGE, which could examine the bacterial composition differences and similarities of agricultural waste composting.Redundancy analysis （RDA） was applied to explore the relationship between bacterial species and composting parameters: pile and ambient temperature, moisture content, pH, C/N ratio, water soluable carbon （WSC）, NH4+-N and NO3--N by using QuantityOne2.0 and Canoco4.5 to determine which combination of variables could explain the species composition differences. The relationship between the ecological distribution of bacterial species and communities with the 8 parameters were clearly revealed on the RDA biplots. The purpose of the redundancy analyses was to determine which combination of parameters explained the most, if any, of the species composition differences of our compost samples. Establishing the identity of bacterial species and composting parameters will greatly enhance our understanding and management of agricultural waste biological treatment systems.The sample,species and parameters biplots were drawn on the results of RDA, and the relationships between the ecological distribution of the bacterial species and communities with the 8 parameters were clearly revealed on these biplots. The composting process could be divided into four phases: mesophilic phase （days 02）, thermophilic phase （days 311）, temperature falling phase （days 1228） and maturation phase （days 1936）. Parameters that affect bacterial distribution and dynamic successions are listed as the following order: NO3--N> pile temperature> WSC> C/N> NH4+-N> moisture content> pH> ambient temperature. It was found that pile temperature, WSC, NO3--N and NH4+-N （P<0.01） and C/N, pH （P<0.05） were the most important parameters significantly affecting the variation of bacterial species in agricultural waste composting. Establishing the correlation between species data and composting performance will greatly enhance the understanding and optimization of agricultural waste composting system.

Related Dissertations

  1. Research on the Laws of Nitrogen and Phosphorus Loss and Control Scheme in Eryuan Rural,X712
  2. Study on the Three-stage Inoculant and Lignocellulose Gegradation in Dairy Manure Composting,S141.4
  3. Effect of Turning ,covering Techniques and Season on Trough Composting,S141.4
  4. Dewatering and Aerobic Composting of Cyanobacteria from the Taihu Lake,S141.4
  5. Heat Change and Wanter Reduction of Cattle Manure in Aerobic Composting,S141.4
  6. The Survey on Food Enterprise Employee-cognitive Levels and Customer-satisfaction Degrees,F224
  7. Alleviating Effects of Bacillus Subtilis on the Cucumber under Salt Stress in Substrate Culture,S642.2
  8. Investigation and Analysis on the Current Situation of the Selection of Raw and Formula of for Plant-Scale Composting,S141.4
  9. The Optimizing Study on Aerobic Composting of Dairy Farm Dejecta,S141.4
  10. Heavy Metal Stress sea state Elsholtzia rhizosphere bacterial community structure and domain -specific gene expression studies,X173
  11. Exogenous agents on the impact of pig manure and chemical fertilizers with organic manure and fertilizer efficiency study,S141.4
  12. The Related Factor Analysis of Lateral Lymph Node Metastasis in Mesolow and Low Site Rectal Cancer,R735.37
  13. Development and Validation of Multivariable Predictive Models to Distinguish Viral Meningitis from Other Infectious Meningitis in Adults,R512.3
  14. Variation of Bacteria Community Stuctures in Eutrophic Water Body During Water Bloom,X524
  15. Study on Endophytic Insecticidal Activity and Diversity of Achnatherum Inebrians,S482.3
  16. Bisporus new model of composting,S141.4
  17. The Clinical Features and Evaluation of High-Risk Osteosarcoma under. the Neoadjuvant Chemotherap,R738.1
  18. Analysis on Influencing Factors of Survival Rate of Replantation of Severed Cut-fingers with Logistic Regression,R658.1
  19. The Growth Pattern and Influencing Factors for the Recurrenced Hepatic Hydatid Disease,R657.3
  20. Relationship Between Polymorphism of IL1β Gene and Susceptibility to Chronic Obstructive Pulmonary Disease,R563.9
  21. The Study of High-tech Sector Investment Value Based on the Multivariate Analysis,F276.44;F224

CLC: > Agricultural Sciences > Agriculture as the foundation of science > FERTILIZERS > Farm manure > Compost, compost
© 2012 www.DissertationTopic.Net  Mobile