Dissertation > Excellent graduate degree dissertation topics show

The Biological Safety Evaluation of Nano-copper/low Density Polyethylene Composite Intrauterine Device

Author: LiJun
Tutor: ZhuChangHong
School: Huazhong University of Science and Technology
Course: Obstetrics and Gynaecology
Keywords: nano-copper/low density polyethylene composite leaching liquors cytotoxicity MTT SEM extract cutaneous stimulation healthy white rabbits maximum dose method guinea pig muscle embedding test
CLC: R318.08
Type: Master's thesis
Year: 2009
Downloads: 78
Quote: 0
Read: Download Dissertation

Abstract


PartⅠExperiment in vitroExperiment one Study of the Cytotoxicity of nano-copper/low density polyethylene composite intrauterine deviceObjective: To evaluate the cytotoxicity of nano-copper/low density polyethylene composite with mouse fibroblast cells.Methods: According to the experimental principle and method of biological appraising standard (GB/T 16886) on medical apparatus, leaching liquors of nano-copper/low density polyethylene composite and TCu220C were prepared with simulated uterine solution. The mouse fibroblast cells (L-929) were cultured with leaching liquors. The proliferation of L-929 cells was measured by tetrazolium colorimetric assay (MTT), and the morphology of the cells were observed by scanning electron microscope (SEM).Results: The relative growth rate of L-929 cells showed significant difference between nano-copper composite group and TCu220C group. The cytotoxicity of nano-copper composite group was 1, but the TCu220C group was 4. The morphology of L-929 cells was normal and the proliferative number of L-929 cells were increased in nano-copper composite group during culture period. The number of L-929 cells were decreased obviously and the morphology of most cells was damaged in TCu220C group.Conclusions: Nano-copper/low density polyethylene composite IUD present no cytotoxicity.PartⅡExperiment in vivo Experiment two Cutaneous stimulation test of nano-copper/low density polyethylene composite intrauterine deviceObjective: To evaluate the cutaneous stimulation of nano-copper/low density polyethylene composite intrauterine device.Methods: According to the experimental principle and method of biological appraising standard (GB/T16886) on medical apparatus, leaching liquors of nano-copper/low density polyethylene composite and TCu220C were prepared with simulated uterine solution. The extracts of two materials were used to stimulate the regional skin of healthy white rabbit spine. The skin stimulus reaction was observed within 72 hours after stimulation.Results: Animals had no exceptional actions during experimental period. No erythema, eschar or edema appeared in the regional skin of rabbits within 72 hours after being stimulated by the extracts of two materials. According to the standard of skin reaction of GB/T16886, the score of both IUDs in this test were 0.Conclusions: Nano-copper/low density polyethylene composite IUD present no skin stimulation.Experiment three Skin sensitization test of nano-copper/low density polyethylene composite intrauterine device Objective: To evaluate the skin sensitization of nano-copper/low density polyethylene composite intrauterine device.Methods: According to the experimental principle and method of biological appraising standard (GB/T16886) on medical apparatus, leaching liquor of nano-copper/low density polyethylene composite was prepared with simulated uterine solution. The extract was used to stimulate the regional skin of guinea pig by maximum dose method which contained initial induction and challenge phases. The skin sensitization reaction was observed at 24h and 48h after challenge phases.Results: Edema and erythema in the skin of guinea pig were observed at 24h in positive control group(1% 2-4-dinitrochlorphene), but those reaction was palliated at 48h. According to the standard of skin reaction of GB/T16886, positive control group was in 2~4 grade, and the allergy rate was 100%. Edema and erythema in the skin of guinea pig were not observed at 24h and 48h in nano-copper composite group and blank group (simulated uterine solution).Conclusions: Nano-copper/low density polyethylene composite IUD present no skin sensitization.Experiment four Muscle embedding test of nano-copper/low density polyethylene composite intrauterine deviceObjective: To evaluate the biocompatibility of nano-copper/low density polyethylene composite with myoideum.Methods: According to the six part of biological appraising standard (GB/T16886) on medical apparatus, two materials of copper/low density polyethylene nanocomposite and TCu220C were inserted into myoideum after sterilization. The reaction of myoideum surrounding the embedding part was observed at the first week, the fourth week and the twelfth week by macroscopic observation and tissue slice observation.Results: Animals had no exceptional actions during experimental period. The wounds during embedding stage had no manifestation of flare and diapyesis. In two groups, the myoideum was showed serious inflammatory reaction and capsule walls were not formed at first week. At fourth week, inflammatory reaction of the myoideum palliated, and fibrous capsule was formed. The inflammatory reaction of the myoideum and fibrous capsule were both inⅠgrade at the twelfth week.Conclusions: Nano-copper/low density polyethylene composite present no adverse reaction in myoiduem. It proved good histocompatibility.

Related Dissertations

  1. Design, Synthesis and Biological Evaluation of Novel Podophyllotoxin Derivatives as Antitumor Agents,R284
  2. Synthesis and properties of new functionalized graphene oxide drug carriers,TQ460.4
  3. Studies on Sex Pheromone Communication System in Orthaga Achatina Butler,S763.3
  4. The Empirical Research on Higher Educational Scientific Technological Innovation Ability and Regional Economical Development,G644
  5. Experiment Study on the Effect of Hybrid Coat in Closing of Dentinal Tubules,R781.2
  6. Functional Study of Active Sites in Scorpion Insect Toxin BmK IT from Buthus Martensii Karsch,Q51
  7. The Impact of Histone Deacetylase Inhibitor Trichostatin a on Proliferation and Apoptosis of Porcine Granulosa Cells,S828
  8. The Syntheses and Applications of Nanao Cadmium Sulfide Based on the Green Chemical Concept,O614.242
  9. Effect on Quality of Chilled Meat by Irradiation and Hawthorn Flavonoids Extract,TS251.51
  10. Studies on Preparation, Purification of the Sika Deer Placenta Oligopeptide,R284.1
  11. The Peptidoglycan from Lactobacillus DM9811: Isolation Extraction and Immunological Activity,R392
  12. The Influence of Bile Duct Partial Obstruction on Guinea Pigs’ Sphincter of oddi in Early Stage,R657.4
  13. Expression of rBTI-2 and Its Anti-tumor Activity,R730.2
  14. A Preliminary Study on Differentiation of Adipose-derived Stem Cell to Odontoblast-like Cell,R78
  15. Oxya endogenous inhibitory protein isolates SDLH feasibility study as a preservative,TS202.3
  16. Actinidia arguta flavonoids Purification and structural identification,R284
  17. The film screenings customer experience impact on customer loyalty empirical research,J943
  18. Preparation and Evaluation of Dry Powder Inhalation of Extract of Trollius Chinesis Bungle,R283
  19. Synthesis and Anti-tumor Activities of N’-benzylidene-2-(4-oxothieno [2,3-d] Pyrimidin-3 (4H)-yl) Acetohydrazone Derivatives,R96
  20. Research on the Inhibition Effect of Different Preservatives on Staphylococcus Aureus Rosenbach in Cantonese Cured Meat,TS251.1
  21. Studies on Genotoxicity of Two Novel Contraceptive Materials,R114

CLC: > Medicine, health > Basic Medical > Medical science in general > Biomedical Engineering > General issues > Biomaterial
© 2012 www.DissertationTopic.Net  Mobile