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Malignant pleural effusion in primary tumor cells ERCC1 , BRCA1 , the expression of TAUmRNA level of detection and significance

Author: JiangMin
Tutor: QianXiaoPing；LiuBaoRui
School: Nanjing Medical University
Course: Medical Oncology
Keywords: Malignant pleural effusion ERCC1 BRCA1 TAU Molecular markers Platinum Taxane
CLC: R730.4
Type: Master's thesis
Year: 2010
Downloads: 37
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Abstract


The first part of ERCC1, BRCA1 and tau mRNA expression and malignant pleural / peritoneal effusion primary tumor drug sensitivity of cells Objective To study the relationship between ERCC1 (excision Repair cross-complementing 1 excision repair cross-complementing gene 1), BRCA1 (breast cancer susceptibility gene, the breast cancer susceptibility gene 1) and TAU (mi-crotubule-associated protein, microtubule-associated protein) expression in the primary tumor cells of malignant pleural effusion, these genes in platinum, Taxol chemotherapy predictive role. Methods 26 patients with pathologically confirmed malignant pleural effusion isolated primary tumor cells, the CCK8 vitro drug sensitivity test for detection of cisplatin, docetaxel anti-tumor effect of primary tumor cells. Drug efficacy in fluorescence quantitative RT-PCR to detect cell gene ERCC1, BRCA1, tau mRNA of relative expression levels. ERCC1 mRNA expression levels in malignant pleural effusion primary tumor cells with platinum drug sensitivity was negatively correlated with high expression of sensitivity to platinum (P = 0.01, r = 0.499). BRCA1 mRNA expression levels in malignant pleural effusion primary tumor cells with cisplatin drug sensitivity was negatively correlated with low expression of TAU and BRCA1 mRNA high sensitivity to cisplatin (P = 0.03, r = 0.440); Primary malignant ascites tumor cells the expression level of docetaxel susceptibility results (P gt; 0.05) Conclusion of ERCC1 and BRCA1 mRNA expression levels may provide a theoretical basis for metastatic ascites and pleural effusion in patients with platinum-based chemotherapy. The second part of ERCC1, BRCA1 and tau mRNA expression containing platinum and taxane chemotherapy in malignant pleural / clinical outcomes in patients with ascites purpose of study malignant pleural effusion Central Plains on behalf of the tumor cells, BRCA1 mRNA expression levels of ERCC1, TAU receiving platinum and paclitaxel chemotherapy in patients with malignant effusion overall survival. Using real-time fluorescence quantitative RT-PCR analysis of primary tumor cells of ERCC1, BRCA1, tau mRNA of the expression levels and compare the expression level for platinum, the relationship between the overall survival of Taxol chemotherapy. Results of 25 cases with malignant pleural effusion in patients with platinum-based chemotherapy in BRCA1 and ERCC1 mRNA relative to the reference gene beta-actin expression level of the median value were 0.980 and 1.952, BRCA1, ERCC1 mRNA expression in patients with malignant pleural effusion clinical the characteristics no correlation. BRCA1, ERCC1 mRNA expression levels of overall survival was not statistically significant (P = 0.389 and 0.057, respectively). COX multivariate regression analysis showed that BRCA1, ERCC1 mRNA expression of malignant pleural effusion overall survival in patients was not statistically significant (P = 0.919 and 0.624, respectively). 23 cases of malignant pleural effusion in patients with taxane chemotherapy in BRCA1, TAU mRNA relative to the reference gene beta-actin expression level of the median value of 1.352 and 7.362, respectively. BRCA1, tau mRNA expression was no correlation with the clinical characteristics of the patients with malignant ascites and pleural effusion. BRCA1, tau mRNA expression level of impact on overall survival was not statistically significant (P = 0.098 and 0.405, respectively). COX multivariate regression analysis showed that BRCA1, TAU mRNA expression level of the impact on overall survival was not statistically significant (P = 0.940 and 0.178, respectively). Conclusion malignant pleural effusion primary tumor cells, BRCA1 of ERCC1, tau mRNA expression levels of the receiving platinum and paclitaxel-based chemotherapy in patients with malignant effusion overall survival was not statistically significant.

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