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Objective observation of esophageal squamous cell carcinoma and normal esophageal mucosa cyclooxygenase -2 (COX-2), vascular endothelial growth factor (VEGF) expression, analysis of esophageal squamous cell carcinoma, COX-2, VEGF expression relations with clinicopathological characteristics and the effect of radiotherapy in esophageal cancer; exploring predict radiosensitivity of patients with esophageal cancer indicators provide the basis for the development of a reasonable individualized treatment program. Materials and methods to collect 60 cases esophagoscopy biopsy before radiotherapy for esophageal squamous cell carcinoma tumor tissue and 20 cases of normal esophageal mucosal tissue, application of SP immunohistochemical method to detect tissue COX-2, VEGF protein expression. 6MV-X line linear accelerator line conventional fractionated radiation therapy within 3 months after the end of radiotherapy and evaluate the efficacy of the evaluation criteria with reference to X-ray film used in patients with esophageal cancer. Χ2 test, Spearman rank correlation and logistic regression analysis and statistical methods such as analysis of COX-2, VEGF expression and clinicopathological characteristics and radiotherapy efficacy relationship. Results 1, COX-2 and VEGF expression in esophageal 60 cases of esophageal carcinoma, COX-2 positive expression rate was 68.3%, 76.7% of VEGF positive expression; 20 cases of normal esophageal mucosa of COX-2, VEGF positive expression rates were 0 and 11.1%; strength of both esophageal carcinoma was significantly higher than the normal esophageal mucosa, the difference was statistically significant (P lt; 0.05). In 60 cases of esophageal carcinoma, COX-2 and VEGF positive expression 35 cases, eight cases of total negative expression, by statistical display both the expression of a significant positive correlation. 2, COX-2, VEGF and esophageal cancer clinical pathological features of COX-2 expression in esophageal cancer patients gender, age, clinical stage, pathological degree of differentiation, depth of invasion, and lymph node status had no significant correlation ( P gt; 0.05). VEGF expression in esophageal regional lymph node status was positively correlated with high VEGF expression of regional lymph node metastasis group without regional lymph node metastasis. Independent of the expression of VEGF and the patient's gender, age, clinical stage, esophageal degree of infiltration and tissue differentiation degree (P gt; 0.05). 3, COX-2 and VEGF expression and the relationship of the efficacy of radiotherapy in 60 patients with esophageal cancer, COX-2 expression the negative group radiotherapy effective rate was 94.7%, the expression of COX-2 positive group radiotherapy effective rate of 61.0%, the statistics analysis, χ2 = 7.288, P = 0.007, with a significant difference. Expression of VEGF negative group radiotherapy effective rate was 100.0%; radiotherapy effective rate of 63.0% VEGF positive expression group. Statistically χ2 = 5.514, P = 0.019, the difference was statistically significant, multivariate analysis of esophageal cancer radiotherapy Logistic regression analysis showed: clinical stage, COX-2 expression, VEGF expression and radiotherapy-related OR values ??were 16.518,6.978,4.423. Conclusion 1 and COX-2 protein and VEGF protein in esophageal squamous cell carcinoma showed a high expression, no expression or low expression in normal mucosa, suggesting that the two involved in the development and progression of esophageal cancer. 2, COX-2 and VEGF expression in esophageal significant positive correlation. The prompt regulatory relationships between COX-2 and VEGF, COX-2 may induce VEGF expression affects tumor angiogenesis and tumor growth. COX-2 expression in esophageal cancer was no significant correlation of clinical and pathological features. The VEGF and esophageal cancer patients with regional lymph node metastasis, lymph node metastasis, high VEGF expression, suggesting that high expression of VEGF protein as a lymph node metastasis sign. COX-2 and VEGF in esophageal positive expression was negatively correlated with term efficacy of radiotherapy in patients with positive people are not sensitive to the radiation, negative radiosensitive, COX-2 and VEGF reflects esophageal carcinoma sensitive and predictive an important indicator of short-term efficacy of esophageal cancer, by detecting the expression of COX-2 and VEGF can develop individualized radiotherapy accordingly, at the same time provides a theoretical basis for the high expression of COX-2 inhibitors were treated with anti-VEGF treatment combined with radiotherapy . Logistic multivariate regression analysis to display: clinical stage, COX-2 expression, VEGF expression associated with radiotherapy, the related OR values ??were 16.518,6.978,4.423.
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