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Expression and Significance of Human Longevity Assurance Gene 2 (LASS2) in Breast Cancer Tissue and Cell Lines.

Author: LianShiXian
Tutor: LuWeiZuo
School: Fudan University
Course: Surgery
Keywords: LASS2 Breast Cancer Immunohistochemistry Real - time quantitative PCR MCF-7 MDA-MB-231
CLC: R737.9
Type: Master's thesis
Year: 2010
Downloads: 35
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Abstract


Background and Purpose : gene expression in the development of high-throughput sequencing technology for the discovery of new biological molecular markers . Massively parallel sequencing (MPSS) technology is the study of gene expression in the most sensitive technology . MPSS sequencing technology in early research , the Biomedical Research Institute of Shanghai Jiaotong University system , the establishment of normal breast tissue and breast cancer tissue MPSS database , found that the human endogenous longevity assurance gene LASS2 gene in the comparative analysis of the database , differentially expressed in breast cancer tissue . LASS2 gene Pan Hui first discovered yeast longevity assurance gene LAGl homologous genes . It is located on chromosome 1q21, encoding 380 amino acid protein , in vitro the experiment proved LASS2 protein is able to inhibit the proliferation of human hepatoma cell . Mizutani [ 3 ] the reported LASS2 gene overexpression can lead to increased expression levels of ceramide and ceramide in apoptosis of the cancer cells . These facts indicate LASS2 gene may play an important role in the development of human cancer cells . This article explores LASS2 gene expression and significance in breast cancer tissues and cells . Methods : Immunohistochemical analysis of breast cancer tissue the chip in LASS2 protein expression , analysis the LASS2 protein in breast tissue , paraneoplastic tissue, differential expression in normal breast tissue . Using real - time quantitative PCR the detection LASS2 gene expression in the breast tissue , benign breast tumor and normal breast tissue . Using real-time quantitative PCR LASS2 gene expression differences in cell line MCF-7 and MDA - MB -231 cell line . Results of : LASS2 protein is highly expressed in breast cancer tissue , and low expression in normal breast tissue . The lower gene in breast cancer LASS2 expression is highly expressed in normal breast tissue . LASS2 gene expression in low metastatic breast cancer MCF-7 cells are highly metastatic breast cancer MDA-MB-231 cells in 3.3 times . Conclusion of : LASS2 gene regulates breast cancer growth gene LASS2 is a breast cancer gene .

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CLC: > Medicine, health > Oncology > Genitourinary tumors > Breast tumor
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