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Study on Clonality and Loss of Heterozygosity in Pulmonary Sclerosing Hemangioma

Author: ZhangLi
Tutor: ZhangWei;GongLi;LiYanHong
School: Fourth Military Medical University
Course: Pathology and Pathophysiology
Keywords: Monoclonal Sclerosing hemangioma Lung tumors Androgen receptor Phosphoglycerate kinase
CLC: R734.2
Type: Master's thesis
Year: 2010
Downloads: 2
Quote: 0
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Abstract


Objective: loss of the live chimeric androgen receptor (androgen receptor, AR), based on the X chromosome in female somatic cells constitute organization phosphoglycerate kinase (polymorphism of phosphoglycerate kinase, PGK) gene locus clonal analysis techniques and laser microdissection technique to investigate the so-called pulmonary sclerosing hemangioma (pulmonary sclerosing haemangioma, PSH) surface cuboidal cells in the tissue interstitial polygonal cells and cloning of the entire lesion, in order to further clarify the nature of the lesion and the lesions with two The relationship between the cells. Methods: elected the PSH female patients with so-called pulmonary sclerosing hemangioma surgical resection specimens of 29 cases. All specimens were 4% neutral formalin-fixed, paraffin-embedded HE staining. And laser capture microdissection (LCM), respectively, to obtain the entire lesion PSH organization polygonal cells and surface cuboidal cells and around the same size as the normal lung tissue, genomic DNA was extracted. The Bst XI digested with the methylation-sensitive restriction cut the enzyme Hpa Ⅱ digestion, nested polymerase chain reaction (polymerase chain reaction, PCR) amplification of phosphoglycerate kinase (phosphoglycerate kinase, PGK) gene. Hha Ⅰ digestion, PCR amplification of X-linked androgen receptor (androgen receptor, AR) gene. PGK gene polymorphisms and cloning of the amplification product by 2% agarose gel electrophoresis display. AR gene amplification products 8% denaturing polyacrylamide gel electrophoresis, silver staining length polymorphism and cloning. Results: 29 cases of so-called pulmonary sclerosing hemangioma lesions, light microscope showed typical histological features lesions mainly by interstitial circular or polygonal cells and surface cuboidal cells composed to form entities District nipple area. structure of the hardened zone and bleeding District. High magnification visible solid areas were round or polygonal cells, the cytoplasm was eosinophilic or slightly transparent, mitotic rare papillary structure surface coating simple cuboidal epithelium. The clonal test results show that the AR and PGK polymorphisms were: 23 cases (79.3%, 23/29) of the AR gene loci were polymorphic. 7 cases (24.1%, 7/29) the PGK gene loci were polymorphic. After HhaI digested with Hpa II, all have two bands polymorphism specimens in a strip density was significantly diminished or disappeared, and another strip retained prove are hyperplasia. Conclusion: polygonal cells, in the so-called pulmonary sclerosing hemangioma tissue surface cuboidal cells and the entire lesion has a similar monoclonal hyperplasia model, the results show that they may are substantive tumor cells.

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CLC: > Medicine, health > Oncology > Respiratory system tumors > Lung tumors
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