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【Objective】 To investigate the DAPK Ⅰ and RARβ abnormal methylation in cervical cancer and its the methylation detection combined with high-risk HPV detection significance in the early diagnosis of cervical cancer and precancerous lesions. 【Methods】 collection Nantong University Hospital outpatient and ward 48 cases of chronic cervicitis, 28 cases of cervical intraepithelial neoplasia (CIN) and 28 cases of cervical cancer in patients with cervical exfoliated cells, methylation-specific PCR and HPV genotyping them methylation and the detection of HPV infection. Combined with liquid-based cytology screening results, compare different screening methods for cervical lesions in patients with different levels of sensitivity and specificity. [Results] The gene DAPK Ⅰ in cervical inflammation chronic, low-level cervical intraepithelial neoplasia (CIN Ⅰ / CIN Ⅱ), Advanced cervical intraepithelial neoplasia (CIN) and carcinoma in situ (CIN III / CIS), cervical squamous cell carcinoma methylation detection rate were 2.08%, 14.28%, 50.00%, 60.71%. Screening with invasive cervical sensitivity, specificity, positive predictive value, and negative predictive value were 60.71%, 86.84%, 62.96% and 85.71%, respectively. Screening of all cervical cancer and precancerous lesions of the sensitivity, specificity, positive predictive value, and negative predictive value were 46.43%, 97.92%, 96.30% and 61.04%, respectively. Gene RARβ in chronic inflammation of the cervix, the low-level cervical intraepithelial neoplasia (CIN Ⅰ / CIN Ⅱ), Advanced cervical intraepithelial neoplasia (CIN) and carcinoma in situ (CIN Ⅲ / CIS), the methylation detection rate of cervical squamous cell carcinoma were 4.55% 7.69%, 42.86%, and 53.57%, respectively. Screening with invasive cervical sensitivity, specificity, positive predictive value, and negative predictive value were 53.57%, 87.67%, 62.50% and 83.12%, respectively. Screening of all cervical cancer and precancerous lesions of the sensitivity, specificity, positive predictive value, and negative predictive value were 40%, 95.65%, 91.67% and 57.14%, respectively. High-risk HPV types in cervical chronic inflammation, low and medium-level cervical intraepithelial neoplasia (CIN Ⅰ / CIN Ⅱ), Advanced cervical intraepithelial neoplasia (CIN) and carcinoma in situ (CIN Ⅲ / CIS), the rate of infection of cervical squamous cell carcinoma were 33.33%, 50.00 %, 71.43%, 89.29%. Screening with invasive cervical sensitivity, specificity, positive predictive value, and negative predictive value were 89.29%, 55.84%, 43.10% and 93.47%, respectively. Screening of all cervical cancer and precancerous lesions of the sensitivity, specificity, positive predictive value, and negative predictive value were 75%, 66.67%, 72.31% and 69.56%, respectively. The two genes combined with HPV screening for invasive cervical sensitivity, specificity, positive predictive value and negative predictive value of 96.43%, 93.11%, 81.82% and 98.59%, respectively. Screening of all cervical cancer and precancerous lesions of the sensitivity, specificity, positive predictive value, and negative predictive value were 82.14%, 100%, 100% and 82.76%, respectively. [Conclusion] DAPK Ⅰ of retinoic acid receptor β was abnormal methylation of these two genes in chronic cervicitis, cervical cancer and precancerous lesions have occurred. The two gene hypermethylation joint detection with high specificity, and can improve the sensitivity of a single gene methylation detection. Gene methylation detection, screening for cervical cancer and precancerous lesions alone superiority of HPV detection and TCT detection is less than two and two or more joint screening of cervical cancer. , DAPK Ⅰ, RARβ methylation of two genes combined with HPV testing in all joint screening method has the highest sensitivity, specificity, positive predictive value, and negative predictive value, is the best method of screening of cervical cancer and precancerous lesions. .
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