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Objective: This topic use iron-induced PTE model study the PTE in rat brain iron metabolism changes, and give deferoxamine intervention, in order to evaluate whether deferoxamine preventive treatment of PTE. Methods: 1, adult healthy male SD rats 40, were randomly divided into normal control group, surgery control group and the PTE model of 24 h, 5 d, 15 d 5 groups of rats microinjection method PTE model. Observed in each group behavioral and to detect EEG, using flame atomic absorption spectrometry and enzyme-linked immunosorbent assay, and possible role in the PTE perspective iron from iron metabolism. Healthy adult male SD rats 72, were randomly divided into the the PTE model of 24 h, 5 d, 15 d group, DFO pretreatment for 24 h, 5 d, 15 d group and DFO early intervention for 24 h, 5 d, 15 d group and other groups 9, microinjection rat PTE model, and the model postoperative immediately or 12 h after giving DFO intervention, observation DFO whether inhibition of PTE. Results: 1 the PTE model brain iron metabolism changes: In the entire experimental period, the iron content of the brain tissue model injection side downward trend, but it is always higher than the normal control group. 2, deferoxamine intervention after rat attack situation: in the entire experimental period, DFO pretreatment group attack average number of times compared with the model group decreased 71.11%, compared to the two groups have a significant difference (P lt; 0.05). DFO early intervention group average attack compared to the number of model group decreased by 30.31% compared to the two groups was significant difference (P lt; 0.05). The average number of episodes of DFO pretreatment group compared with the DFO the early intervention group, there are significant differences. (P lt; 0.05). 3, after the intervention of deferoxamine iron content changes: 24 h, after the establishment of the PTE model model rat brain iron content higher than the of DFO pretreatment group and DFO early intervention group, the difference was statistically significance (P lt; 0.05) pretreated group of DFO and DFO early intervention group rat brain tissue iron content compared, the difference was not statistically significant. The PTE model to establish 5 d and 15 d, the PTE model group rat brain iron content whether DFO pretreatment group or compared with DFO early intervention group, differences are not statistically significant. 4, deferoxamine intervention transferrin concentration changes: DFO pretreatment 24 h rats transferrin concentration in brain tissue than DFO pretreatment 5 d groups and DFO pretreatment 15 d group, the difference was statistically significant (P lt; 0.05), 5 d rats brain tissue transferrin concentration in 15 d group, no statistically significant difference between pre-treatment with DFO and DFO pretreatment. Experimental point in the DFO the early intervention group transferrin concentration in rat brain tissue gradually increased, the differences were statistically significant (P lt; 0.05). Conclusion: PTE rat brain tissue within the iron content and transferrin protein content increased, prompted iron ions may be part of the of PTE formation and outcomes, and may PTE to form the initiating factor. 2, the use of deferoxamine intervention, can significantly reduce the rat model of brain iron content and number of episodes, especially the pre-treatment is more effective. Can also reduce the content of free iron in the brain tissue, suggesting that DFO can be used as a means of treatment PTE.
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