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Objective To evaluate the fluorescence in situ hybridization (fluorescent in situ hybridization, FISH) technology in prenatal diagnosis , and Preliminary clinical application of FISH technology and its quality control . The method of use of the FISH technique on amniotic fluid samples from 189 pregnant women at 16 to 24 weeks pregnant , 32 cases of pregnant the villus sample line of 25 to 32 weeks of fetal cord blood samples , and five cases of 9 to 12 weeks pregnant chromosome number or microdeletion . 226 cases the samples were routine karyotype analysis . Familiar with the operation of conventional FISH technology based on inspection by the literature , FISH experiments explore the technical aspects of the specimens digestion , hybridization , slides were washed , FISH and explore a complete quality control system , the establishment of this laboratory mature FISH prenatal diagnostic platform . Results 226 cases samples were obtained within 24 to 48 hours FISH diagnosis , diagnostic success rate of 100% . In addition to the two cases of amniotic fluid culture failed , 224 specimens were in the 3 to 4 weeks cytogenetic diagnosis , diagnostic success rate of 99.1% . Normal karyotype of 213 cases , accounting for 95.1% ; abnormal karyotype 11 cases , 4.9%; abnormal chromosome number four cases (1.8%) , chromosome structural abnormalities in 7 cases, accounting for 3.1% . 11 cases of chromosome microdeletions all normal test results . Both methods detected four cases of abnormal number of chromosomes and chromosome unbalanced translocation , and another six cases of abnormal conventional karyotype analysis , FISH method could not be detected beyond the detection range . The two methods of detection results of all the samples are consistent . Discussion FISH experiments to optimize the operation of FISH technique , shorten the detection time to mature in our laboratory FISH for prenatal diagnosis platform . Conclusion FISH to detect short time , and greatly ease the anxiety of pregnant women and their families , and can be applied to a variety of different samples tested , breaking the gestational age limit . Because of its high efficiency , save time , drawing a diverse advantages in prenatal diagnosis has important clinical value .
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