Dissertation > Excellent graduate degree dissertation topics show

A Laboratory Study on the Influence of Coagulation Function in Pregnant Rats Injecting Amniotic Fluid Components

Author: SongZiHeng
Tutor: YuJian
School: Zunyi Medical College,
Course: Pathology
Keywords: Amniotic fluid component Tail vein injection Coagulation function APTT Tryptase Intrinsic coagulation pathway
CLC: D919.1
Type: Master's thesis
Year: 2011
Downloads: 1
Quote: 0
Read: Download Dissertation

Abstract


Objective:This study was designed to establish fast and convenient mode of pregnant mice injected with amniotic fluid components,so as to observe the dynamic changes of the effect of the composition of the amniotic fluid on the coagulation of pregnant mice.And it can supply clues for the possible mechanisms of the effect of the composition of the amniotic fluid.Methods:(1)15 SD rats in late pregnancy with gestational age 21 days, were randomly divided into 3 groups:5 rats for control group (saline group),5 rats for the group after injected amniotic fluid 1 hour,5 rats for the group after injected amniotic fluid group of 2 hour. Amniotic fluid was injected into rat blood circulation respectively through tail vein. The rats were sacrificed in euthanasia after injected 1 and 2 hours respectively. (2) Indicators test:Got blood from right ventricle. Weight the liver, kidney and lung to calculate the organ coefficient. Serum was separated by centrifugal. The secretion of serum tryptase were measured by ELISA, and the international normalized ratio (PT-INR), prothrombin time (PT), activated partial thromboplastin time (APTT), thrombin time (TT), fibrinogen (FIB) were analysed by automatic biochemical analyzer.Result:(1) Compared with the control group, when the amniotic fluid was injected in pregnant mice 1,2 hours, mice lung, kidney, liver, organ coefficient increases, but the difference was not statistically significant. (2) Tryptase expression increased after the amniotic fluid composition injected.and 2 hours is higher than 1 hours.When compared with control group, difference was statistically significant (P<0.05); (3) After amniotic fluid composition injected, the pregnant rats PT, TT, APTT prolonged, while the most obvious is APTT .The difference compared with control group was statistically significant(P<0.05).Conclusion:(1).Tail vein injection is a useful way to establish a rat amniotic fluid model, and minimally invasive surgery can be taken. (2) PT, TT, APTT can be important, constant and reliable indicators for the coagulation, and the dynamic changes occurring at different times.APTT elongation is more definite in this study, suggesting intrinsic coagulation pathway plays an important role in coagulation. (3). Amount raising of tryptase in serum may be a result of intrinsic coagulation pathway activation.

Related Dissertations

  1. Inhibition of Hepatitis B Virus Expression in Mice by Rnai,R512.62
  2. The Clinical Study on the Abnormality of Blood Lipids and Coagulation in Type 2 Diabetic Nephropathy,R692.9
  3. The Role of Mast Cells and Mast Cell Tryptase in the Pathogenesis of Nasal Polyp,R765.25
  4. Study on the Expression of Mast Cell Tryptase in Colonic Mucosa and the Effects of Its Inhibitor in Rats with DSS-induced Ulcerative Colitis,R574.62
  5. Research of Magnetic Liposomes Crossing Blood Spinal Cord Barrier after Spinal Cord Injury in Rats,R651.2
  6. Diarrhea-predominant irritable bowel syndrome ascending colon mucosa tryptase , α1- antitrypsin expression and its significance,R574
  7. The Experimental Study of Xiao Shuan Yin’s Decoction on Hemorheology from the Rabbit’s Model about Deep Vein Thrombosis,R285.5
  8. The Effects of Alcohol on Rats’ Cerebrovascular Ultrastructural and CD146 Protein Expression,R743
  9. The Effects of Ascending Colonic Mucosa Tryptase in Patients with Diarrhea-predominant Irritable Bowel Syndrome on "Ca~2+" i of Rats Dorsal Root Ganglion Neurons,R574
  10. The Study on the Mechanisms of YSH Inhabiting the Renal Interstitial Fibrosis of UUO Rats,R692.9
  11. Antithrombotic Effect of Polysaccharide L01 from Laminaria on Arterial Thrombosis Model in Rabbit Induced by Intravascular Electricity-Stimulus,R285.5
  12. Coagulation and malignant tumors and their metastasis Experimental Study,R73-3
  13. Comparative Analysis of Laparoscopic and Open Gastrointestinal Tumors Surgery for Coagulation Status,R735
  14. Lab Examination of Trytase and Chymase Serum Levels and Their Clinical Significance in Patients with Viral Hepatitis B,R446.6
  15. Inhibitive Effect of Systemic Administration of Avastin on Retinal Neovascularization in the Rat,R774.1
  16. The chitooligosaccharides anti -tumor immune response study,R730.3
  17. Effects of Sry Silencing on Mouse Embryos Sex Determination Genes and Gonad Development,Q78
  18. Detection of Healthy Individuals and Anaphylactic Patients Serum Tryptase Levels,R363
  19. The Therapeutic Effects of Rat IL-10 Plasmid DNA Tail Vein Injection on Hepatic Fibrosis in Rat and Its Mechanisms,R575.2
  20. Purification, Characterization and Pharmacodynamic Activity of Proteases and Its Isoenzyme from Nereis Virens,R96

CLC: > Political, legal > Legal > UNIVERSITY > Forensic > Forensic basic science
© 2012 www.DissertationTopic.Net  Mobile