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Studies on Genetic Transformation of Bt.CPTI Insect-Resistant Gene to Rice

Author: Wang
Tutor: XuZhong
School: Northeast Agricultural University
Course: Botany
Keywords: Agrobacterium-mediated transformation Biolistic Single - cell electroporation Genetic transformation
CLC: S511
Type: Master's thesis
Year: 2003
Downloads: 66
Quote: 0
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Abstract


Rice is one of the most important food crops in the world , half of the population is the staple food of rice worldwide . According to statistics , China the rice acreage accounts for about 28.9% of the global area sown to grain , the total output ranks first of all food crops . Is one of the main food of the people of our country . However , rice production is a serious threat by lepidopteran pests every year due to pest rice yield in more than 10% . The experimental application of insect-resistant gene bivalent insect-resistant genes containing bivalent insect-resistant genes in transgenic plants can delay the pests on transgenic plants to produce anti- toxic , and extend the service life of the genetically modified crops , and give full play to the role of genetically modified crops . Insect-resistant genes used in this experiment bivalent insect-resistant genes through Agrobacterium-mediated transformation , particle bombardment , single-cell electroporation Dongnong 419 rice varieties , Dongnong 420 , Dongsheng 16 Bt gene genetic transformation , explore the co-culture way confrontational callus incidence ; the antioxidant inhibition the transformed callus browning and restore growth state ; different enzymes and osmotic pressure on the single-cell dissociation influencing factors ; established for single-cell electroporation cell suspension culture medium and hormone concentrations ; transgenic plants , GUS and PCR , initially confirmed that the gene has been integrated into the rice genome .

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CLC: > Agricultural Sciences > Crop > Cereal crops > Rice
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