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Research on the Levels and Speciations of Trace Elements in Chinese Herbal Medicine of Veterinary

Author: ZhongJinFang
Tutor: FangShaoXin
School: Northwest University for Nationalities
Course: Clinical Veterinary Medicine
Keywords: Chinese herbal medicines trace element remove blood stasis Speciation Analysis
CLC: R284.1
Type: Master's thesis
Year: 2010
Downloads: 74
Quote: 0
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Abstract


The contents,primary,secondary-level speciation of 5 trace elements (Mg、Fe、Zn、Cu、Mn),which contained in six Chinese herbal medicines for promoting blood circulation and removing blood stasis,was studied for the first time. The contents of 5 trace elements in six Chinese herbal medicines were determined by atomic absorption spectrometry,the relationship between the their effect was discussed. Different elements in Chinese herbal medicines in the presence of different chemical and physical form, the solubility is different from the water and ethanol. The research content of elements in the primary form of traditional Chinese medicine is study of the dissolution rate and dissolve character trace elements in it, in turn, we can know whether the presence of elements in traditional Chinese medicine for the ease of stripping form. In this paper, two methods of trace elements extracted from traditional Chinese medicine were used. One is to use decoction extraction method; another is to use 20% ethanol extraction method.The contents of sample of primary speciation obtained from primary analytical flow were determined by atomic absorption spectrometry,species distributions of the primary speciation analysis,such as extractive rate,immerse-residue ratio etc were also calculated. The concentration of nine element in medicines residue were high,which showed that the residue were still valuable. We can found: Immerse-residue ratio of Stiff silkworm in decoction extraction is greater than 20% ethanol extraction. Especially the extraction rate of Zn ethanol extract of decoction of 16 times; the extraction rate of Fe ethanol extract of decoction extract 25 times. So decoction extraction of Stiff silkworm is more favorable dissolution of trace elements in, to play a better efficacy. Trace elements extraction rate of Safflower in the ethanol is lower than decoction .But the extractive rate of Cu is increase in 20% ethanol. Especially the extraction rate of Zn ethanol extract of decoction of 5 times. The Cu of Safflower maybe exists in the form of organic. On the whole, the use of decoction way better efficacy. Trace elements extraction rate of Suberect spatholobus stem in the ethanol is lower than decoction. Zn elements in the Rhizoma chuanxiong have the higher extraction rate in the water decoction, but Cu, Fe; Mn extraction rate is greater than ethanol extract of decoction. The extraction rate of Fe ethanol extract of decoction extract 3 times. Therefore Chuanxiong medicine Herb plays a better efficacy available ethanol extraction method.Secondary-level speciation of Mg﹑Cu﹑Fe、Zn and Mn in Stiff silkworm、Safflower、Scorpion、Suberect spatholobus stem、Scolopendra and Rhizoma Chuangxiong Herb were studied on the base of the analysis of primary speciation. The results of secondary-level speciation were obtained by using cation-exchange resin,Amberlite XAD-2 macroreticular resin and Chelex-100 chelating resin. Five elements ( Cu、Zn、Fe、Mn、Mg) almost existed on inorganic forms, but the Cu of Safflower exists in the form of organic. The Cu of five Herb existed on no free form, the others existed on free form.

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CLC: > Medicine, health > Chinese Medicine > Of Pharmacy > Traditional Chinese medicine chemical > Chemical analysis and identification
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