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Objective: To study the TMP anti- atherosclerosis ( AS ) , pharmacodynamics and molecular mechanisms , to further explore the impact on the of AS pathogenesis of the abnormal endothelial function , for expand Tetramethylpyrazine the clinical indications , the AS early diagnosis and type and anti- AS clinical Evaluation of Drugs to provide new ideas and new strategies . METHODS : In 12 weeks of high-fat diet - induced rabbit AS model , evaluation ligustrazine on the film thickness of the aortic plaque area , intima / media ratio , intimal foam cell count , HE staining aortic morphology and lipid levels impact ; further ox-LDL -induced primary cultured human coronary artery endothelial cells , the effect of TMP chemokine MCP-1, adhesion molecules ICAM - 1 and inflammatory factors in the COX-2 gene and protein expression ; research coupled NF - kappaB signaling pathway activation in them ; proteomics , combined with mass spectrometry and bioinformatics , protein profiling study differences TMP anti- ox-LDL -induced endothelial cell injury early formation of AS expression and for functional verification . : TMP can reduce plaque area , the thickness of the intima / media ratio , intimal foam cell count ; light microscope after HE staining , the ligustrazine reduce aortic intimal thickening extent , reduce foam cell aggregation and maintain the basic integrity of the membrane and smooth muscle structure , reducing endothelial cell loss , TMP can improve the morphology of the aortic disorders ; TMP can also reduce blood lipid levels to maintain the normal physiological function of the endothelial cells . The ligustrazine inhibit chemotactic factor MCP-1, adhesion molecules ICAM -1 and inflammatory factors in the COX-2 gene and protein expression , inhibition of NF - kappaB nuclear coupling shift . The functional proteomics found 12 differentially expressed proteins , its terminal amino acid sequence were identified by the LC-ESI-MS/MS tandem mass spectrometry , and bioinformatics database searches to determine its biological function : energy metabolism related proteins , heat shock proteins , receptor tyrosine kinase . Conclusion: the ligustrazine targeting endothelial cells intervention the AS early part of the initiating , inhibit the formation of foam cells produce anti- AS ; characteristics of multi- target , multi-signal pathway is involved in its new role .
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