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Mesenchymal stem cells are bone marrow-derived multipotent differentiation potential of stem cells, under certain conditions, can be induced to differentiate into osteoblasts, chondrocytes, adipocytes, muscle cells, heart cells, while it easily obtained, in rapid expansion when cultured in vitro, can be autologous, bone, cartilage, heart and other tissue repair and reconstruction of cell sources. In this experiment, rabbits research object, rabbit bone marrow mesenchymal stem cells with 5 - azacytidine induction, in vitro and in vivo differentiation of cardiomyocytes directed case is damaged myocardial tissue cell transplantation research foundation. The experiments were performed in the following areas of: 1, through repeated practice, worked out relatively simple, efficient and practical rabbit bone marrow mesenchymal stem cells isolated and cultured technology: bone marrow cells gradient centrifugation of bone marrow mesenchymal stem cells, the resulting cells by flow cytometry to monitor surface antigen expression, the results showed that the cell uniformity is better, negative for CD34 cell surface antigen, CD44 expression was positive, but also further identified cells obtained high purity. 2, in vitro experiments: the rabbit bone marrow mesenchymal stem cells as seed cells for 5 - azacytidine induction, then take the cells were cultured 7,14,21,28 d immunohistochemical staining to detect myosin heavy chain (myosin heavy chain). Results The group of cells cultured 21,28 d immunohistochemical staining was positive, while the experimental group, the cells cultured 7,14 d and time control of the various cell lines were negative immunohistochemical staining was confirmed by 5 - azacytidine-induced bone marrow mesenchymal stem cells can Myocardial Fourth Military Medical University master's degree thesis like differentiation. Meanwhile, the results show the 5 - aza cell celecoxib optimal induction time for the first 72 hours, optimal induction concentration 10umol few. 3, in vivo experiments: the rabbit bone marrow mesenchymal stem cells were grown in cell for 5 - aza celecoxib induced autologous cells transplanted into infarcted myocardium, and further improve the bone marrow mesenchymal stem cell differentiation of cardiomyocytes. The results showed: Bone marrow mesenchymal stem cells in the body to continue cardiomyocytes differentiation and cell fusion with host myocardium repair of myocardial injury; while in repairing damage, bone marrow mesenchymal stem cells can also promote peripheral vascular regeneration. The study showed that: In this study, bone marrow derived, isolated and cultured bone marrow mesenchymal stem cells, after 5 - aza cells after celecoxib induced to differentiate myocardial samples for tissue engineering to find a new seed cells, while autologous Bone marrow stromal stem cells as seed cells in vitro directed differentiation on the basis of myocardial tissue engineering (myocardial regeneration) research, and with the combination of clinical micro-injection techniques to the treatment of ischemic heart disease, and further improve the bone marrow Mesenchymal stem cell-like differentiation of cardiomyocytes for the treatment of heart failure provide a new means for the experimental basis for clinical application.
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