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Study of Effective Substances Screening for Flos Lonicerae Based on the "Spectrum-effect" Combination

Author: WangLei
Tutor: YuanHaiLong
School: Chengdu University of Traditional Chinese Medicine
Course: Pharmacy
Keywords: Honeysuckle Antioxidant activity Fingerprinting Biothermodynamics Effective substances screening Spectrum activity relationship
CLC: R284.1
Type: Master's thesis
Year: 2010
Downloads: 229
Quote: 0
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Abstract


Honeysuckle plant honeysuckle Caprifoliaceae dried buds or flowers, with the early opening is commonly used in Chinese traditional medicines, is clinically recognized as a common and effective traditional Chinese medicine. Separation of traditional and modern analytical methods, combined with the characteristics of high-throughput, trace vitro antioxidant activity of substance evaluation method DPPH and FRAP method evaluation components antioxidant activity. Measurement results according to the the UPLC spectrum antioxidant activity \The results showed that the antioxidant activity of substances containing mainly the peak time for the two peaks 4.44,5.14 min, the material component showed a strong antioxidant activity, and preliminary judge for chlorogenic acid compounds. Research honeysuckle herbs the UPLC chemical fingerprint, the the different origin honeysuckle Lonicera confusa fingerprints. The system examines the impact of various factors on the development of honeysuckle Herbs UPLC fingerprint. Using similarity criteria as a fingerprint, calibration characteristic peaks, to establish its control fingerprint, Total mode reference by \The evaluation results. Fingerprint cluster analysis results of 20 samples are divided into two categories, the first category honeysuckle Herbs, Lonicera confusa class herbs. Honeysuckle herbs produced in the main producing areas as the research object, the application of micro-calorimeter, measured 37 ° C under the conditions of E. coli under normal conditions and honeysuckle role thermogram and kinetic model fitting, get a series of thermodynamic parameters. Honeysuckle thermogram through principal component analysis, the first exponential phase growth rate constant K1, the first exponential phase maximum exothermic peak time T1 and the second exponential phase power P2 in the overall reflected the honeysuckle biological thermally active The difference is particularly important. The three parameters and the chemical fingerprint of the peak area of ??canonical correlation analysis, the results show the strength of the bio-thermal activity of different origin honeysuckle is mainly affected by the fingerprinting of 3,8,9 peak impact. This study provides a new way for Chinese medicines substances, the upcoming conventional chemical, biological, thermochemical or pharmacology combined \to promote the development of China's pharmaceutical industry.

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CLC: > Medicine, health > Chinese Medicine > Of Pharmacy > Traditional Chinese medicine chemical > Chemical analysis and identification
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