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Studies on Chemical Constituents and Activities of Orobanche Cumana Wallr

Author: ZhaoMengXia
Tutor: YangChao
School: Harbin Institute of Technology
Course: Physical and chemical
Keywords: Orobanche cumana Wallr. chemical compounds Phenylethanoid Glycosides antioxidant activity antibacterial activity
CLC: R284
Type: Master's thesis
Year: 2012
Downloads: 0
Quote: 1
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Abstract


Orobanche cumana Wallr.(Orobanchaceae) widely distributed in northeast andnorthwest of China, is one of the important medicinal value plants. Compared withthose of the control of its harmness, there were less studies on the isolation andbioactivites of the chemical constituents of Orobanche cumana Wallr. In this thesis, theisolation, identification and bioactivities of a series of compounds and crude extractsfrom different parts of it have been studies.There were30compounds isolated from Orobanche cumana Wallr. via columnchromatography, preparative thin-layer chromatography, Sephadex LH-20andODS-A. Chemical structures of20compounds of them were illustrated based on thephysicochemical properties, GC-MS, NMR and HRESIMS. Moreover,15compoundswere first obtained from this plant.Bioactive assays mainly contain antioxidant activity test and antibacterial assay.Antioxidant activities of crude extracts from different parts of Orobanche cumana Wallr,4kind of compounds were evaluated by ABTS, DPPH and FRAP assays, respectively.Through the test, we got the comparison of antioxidant activities values of them.Moreover, the antibacterial activities on Staphyloccocus aureus, Escherichia coli and P.Aeruginosa of crude extracts from various polarity segments were tested based on agarslab ditch method. We believe that this test can supply a certain theory of supplementaryfor Orobanche cumana Wallr.

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CLC: > Medicine, health > Chinese Medicine > Of Pharmacy > Traditional Chinese medicine chemical
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