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Study on the Effect of Propofol on the Discharge of Inspiratory Neurons in the in Vitro Brainstem Slice from Neonatal Rat and Its Possible Mechanisms

Author: Shi
Tutor: WuZhongHai
School: First Military Medical University
Course: Neurophysiology
Keywords: Propofol Medulla oblongata Medial area of nucleus Basic rhythmic breathing Inspiratory neurons Respiratory center γ- aminobutyric acid A the receptor (GABA_A receptor ) Glycine receptor (Gly receptor ) Inspiratory time
CLC: R965
Type: Master's thesis
Year: 2003
Downloads: 18
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Abstract

Purpose of 1) application of the neonatal rat brainstem slice specimens were simultaneously recorded the sublingual lining root and facial nerve medial region of nucleus (the medial area of ??the retro facialis T nucleus of of respiratory neurons mNRF) units discharge activities observed concentrations of propofol (2,6-diisopropylphenol, propofol) inspiratory neurons from the role of the respiratory center of the medulla oblongata law. 2) application of γ-aminobutyric acid A the receptor (GABA_A receptor) and glycine (Glycine, Gly) receptor antagonist, observation of propofol inspiratory neurons discharge activities, and further explore the effects of propofol on The bulbar respiratory rhythm central inspiratory lining the possible mechanisms of the neurons. Methods newborn SD rats (0-3d), male or female. Reference methods and improved Suzue production record hypoglossal nerve discharge from the brainstem slice specimens as indicators for monitoring of respiratory activity and simultaneously recorded unit discharge activity of inspiratory neurons in the ventrolateral medulla. Kreb's solution (the modified Kreb's solution, MKS), improved irrigation the displaced brainstem slice experiments were randomly divided into Ⅰ ~ Ⅶ groups (each n = 6). Ⅰ group was the control group (MKS group), group Ⅱ ~ Ⅴ propofol concentration were 5μmol / L, 20μmol / L, 50 μmol / L, 100 μmol / L perfused 3min first Ⅵ the group to GABA_A receptor specificity blocker bicuculline (Bicuculline, 20μmol / L) with propofol 20μmol / L, of Ⅶ groups to glycine receptor antagonist disabilities Ning (STR, 100 μmol / L) were observed after administration of 1,3 5, 10, 15, 30 min inspiratory neurons discharge duration and peak expiratory drive (inspiratory neurons discharge quiescent) and respiratory rate changes. 1) I group the parameters of each time point had no significant changes (P> 0.05). 2) Section II the ~~ Ⅴ group inspiratory neurons discharge within 1 ~ 30min away both showed significantly reduced gradually, 15min when the role of the most significant and groups perfused with MKS blank compared to the control group, there were significant differences . 3) Group Ⅱ ~ Ⅴ 1min expiratory duration no statistically significant changes in 30min within breath away were shown to significantly prolong 15min within reach its maximum effect. 4) in each group at 1 ~ 3min inner suction neurons discharge peak increase of 10 to 30min within phase change in the discharge peak before administration, but the peak value of the discharge time of the 1-3min ratio significantly decreased, was to acetyl first state plus mediated _ after the array low tendency among groups discharge peak No significant difference. 5) groups in three 3omil: inside the mouth suction frequency gradually slowed down to 5 a clever min and reached its maximum inhibitory effect. The group l sentence changes in respiratory frequency was no significant difference. 6) bicuculline propofol perfusion group 10 of the inspiratory time and expiratory time to the sub-intestinal after there was no significant difference. Before and after administration of 7) Guests Ning propofol perfusion group inhale!! Inch away, '(significant difference expiratory duration after administration IOmin 15mll7, 3Omi when compared to pre-administration There are significant differences. Conclusions: These results suggest that: 1) propofol inhibit neonatal rat medullary inspiratory neurons in brain slices specimens discharge process, mainly for the discharge of inspiratory neurons quiescent when The process was prolonged, this inhibition in a concentration-dependent manner. 2) propofol can make breathing frequency reduce administration time delay China, but this inhibition was not significantly inhibited discharge peak inspiratory neurons. 3) GABAA receptor-mediated the propofol suction left to discuss the discharge activity by the respiratory center of the medulla oblongata} _lrJ inhibition. 4) glycine receptor may also participate in the propofol medulla oblongata China this r. \

Study on the Effect of Propofol on the Discharge of Inspiratory Neurons in the in Vitro Brainstem Slice from Neonatal Rat and Its Possible Mechanisms

Abstract

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