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Purpose: Observation a long time to fill the stomach for rats and force the toxicity level, poisoning the target organ, and after the recovery of sex and delay. in clinical research and applied to provide toxicology basis.Material and method:Material:The reference to Bliss method. Rats were divided into nasal capsule Bilibao high, medium and low-dose group, control group, administered orally for 4 weeks, withdrawal recovery 2 weeks. Observe the behavior of rats during the experiment activities, appearance signs, body weight, food intake changes, administration and the restoration of the end of the end of the two anatomy, routine blood testing, blood biochemistry, organ coefficient. Observe the pathological changes of tissues and organs.Results:The nasal capsule half of the lethal dose of Bilibao 128.8g/kg, 95% on average CI: 119.3 ~ 139.1g crude drug / kg; long-term toxicity experiments,Bilibao capsule of rat oral administration of high doses of nasal capsule is equivalent to Bilibao Pharmacognosy 30g / (kg ? d), no obvious toxicity. the preparation of rats, signs and weight and a quantity of food and viscera coefficient ; no obvious impact of rats in the blood and blood of biochemistry no obvious impact indicators ; pathological check the results showed that in the organs of the organization without toxicity.Conclusion:Respectively to fill the stomach of a big nose, the nippon extract 5g 15g, 30g kg d ( ) have medicine for four weeks, not in the preparation of the obvious toxicity.The nasal capsule Bilibao safe and reliable.
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