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Finger Print Reserch and the Principal Componds Study of Garlic

Author: WangXu
Tutor: ChenJian；LiXinXia
School: Xinjiang Medical University
Course: Drug analysis
Keywords: Fingerprint Alliin Allicin Garlic-Polycose
CLC: R284.1
Type: Master's thesis
Year: 2010
Downloads: 75
Quote: 0
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Abstract


Objective: 1. Allium sativum L belongs to Liliaceae genus Allium. Set up TLC fingerpint of garlic aminoacids can offer a parameter for controlling or estimating the quality of garlic. 2. To check up and the test methods of sign component in US Pharmacopeia and Europe Pharmacopeia,try out the test to same object according to these Pharmacopeias and anlysis the result, we can get the difference, which can help us to get precise result in the further study. 3. Study the physico-chemical property of garlic-polycose, to control the quality of garlic-polycose products. Method: 1.Line up the different processing and developing solvent in reference, screened the optimizing cooperation as the fingerprint analytical method. Try out the analysis method to 12 species of garlic which major come from Xinjiang, after scaning the tracks and getting fingerprint, use similaryity software to analysis. 2.Use HPLC to experiment the different test in USP Pharmacopeia and Europe Pharmacopeia, and contrast the relationship of these results. 3. Use UV, refractometer, viscometer, and HPTLC to analysis the saccharinity, viscosity, solid object content, and the type of suger of 3 batches of garlic-polycose. Result: We get the similaryity, Rf of the 12 species of garlic and the ratio of alliin in all amino acids, and find the ratio of Xinjiang Aheqi is highest. We can get a higher Allicin content when use the method in American Pharmacopeia, and the allicin contents are higher then evaluation by all different purity alliin raw materials except 43.9%, and the high degree is increasing along with higher purity of raw material. In the other hand, we can get a lower Allicin content when use the method in European Pharmacopeia, and the allicin contents are lower then evaluation by all different purity alliin raw materials except≥96%, and the differences grow down as the purity of raw material increasing.

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CLC: > Medicine, health > Chinese Medicine > Of Pharmacy > Traditional Chinese medicine chemical > Chemical analysis and identification
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