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Follicle stimulating hormone (FSH) products used in livestock production from the pituitary gland of cattle , sheep, pigs and other livestock extracted directly , due to limited sources of material purity and often the pollution , the impact of other hormones FSH widely used . Using genetic engineering methods to mass production of high purity FSH and reduce the production cost , and this to the livestock production has an important significance . This test is a new female goat slaughtered pituitary extraction of total RNA was reverse transcribed to obtain cDNA, this cDNA as a template , PCR - amplified gene fragments , respectively, to obtain the long- 360bp of goat FSH - α subunit of DNA fragments and long the goat FSH-β subunit of 390 bp DNA fragment , with the intended purpose of the gene fragment of the same size. They were cloned to pMD 18-T, the random selection of a few positive recombinants were sequenced . Sequencing results with sheep and other mammals , the gene and the corresponding amino acid homology comparison and analysis . Show of goat FSH- α , β subunit genes and their respective corresponding amino acids and other mammals homology are very high , generally in the 80% or more , and verified in the same , even in all mammals . FSH-α subunit is extremely conservative ; FSH-β subunit is species-specific , but is quite conservative . The resulting of goat FSH- α , β - subunit gene were used to increase the corresponding restriction endonuclease sites primer amplified recovery connection with pMD 18-T , screened positive recombinants , a large number of double digestion recovering the of goat FSH- α with sticky ends , the β -subunit gene , the plasmid with sticky ends respectively double digested with the same two enzymes recovered PF connected , and screening for positive recombinants . This is the way to build FSH single subunit of eukaryotic expression vector gFSH - α -PF gFSH - β - PF and FSH the dual subunit of eukaryotic expression vector gFSH - α - β -PF , dFSH -α - gFSHβ -PF . This study is the first goat FSH cloning, sequence analysis and expression vector was constructed aspects of exploration , to fill the blank of domestic and international restructuring goat FSH basic research , and provides a theoretical basis and experimental evidence for mass production applications for the future reorganization goat of FSH .
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