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The Electrochemical Study for the Anticancer Drug and the Interaction of Anticancer Drug with DNA

Author: WangCuiHong
Tutor: YeBaoXian
School: Zhengzhou University
Course: Analytical Chemistry
Keywords: Electrochemical Study Anti-cancer drugs The wax dipped graphite electrode Detection limit Azaguanine Hanging mercury drop electrode Electrochemical Properties Voltammetric behavior Phosphate buffer solution Electrochemical methods
CLC: O657.1
Type: Master's thesis
Year: 2003
Downloads: 139
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Abstract


As we all know, the material basis of life information transfer deoxyribonucleic acid (also known as DNA), the destruction of the DNA molecule is bound to create obstacles to the process of life, or even interrupt. Thus, for the study of DNA is currently the focus of attention. Tumor disease is caused by DNA damage, the number one killer of threat to human life, naturally became the goal of the study of the scientific community. In this thesis, electrochemical methods studied two anti-cancer drugs - 8 - azaguanine and 6 - mercaptopurine electrochemical properties, and its interaction with DNA to do a preliminary study. The main content of the paper include the following aspects: 1: The effect of anti-cancer drugs 8 - azaguanine (8-AG) oscillopolarographic analysis methods. In 0.10mol/LH 2 SO 4 base solution, 8 - azaguanine at-1.03V (vs SCE) a sensitive reduction wave, peak current 8 - azaguanine concentration of 1.00 × 10 -8 mol / L to 9.00 × 10 -5 mol / L range a good linear relationship, the detection limit up 9.00 × 10 -9 mol / L. The method is simple, high sensitivity, accuracy, recoveries were 95.0% ~ 103%. 2: 8 - azaguanine (8-AG) in wax dipped graphite electrode electrochemical properties. HAc-NaAc buffer solution, 8-AG in wax dipped graphite electrode on a diffusion controlled oxidation wave peak potential of 1.20V (vs SCE), the peak current and the 8-AG concentration in 8.00 × 10 -7 mol / L ~ 1.00 × 10 -4 mol / L range, a good linear relationship of the detection limit of 5.00 × 10 -7 mol / L. The electrode reaction were studied in detail using a variety of electrochemical techniques to obtain the corresponding kinetic parameters, create a sensitive, rapid and 8-AG detection method. 3: HAc-NaAc (pH3.60) buffer solution 8 - azaguanine (8-AG) and double-stranded DNA interactions in the the wax dipped graphite electrode voltammetric behavior. In the wax dipped graphite electrode, 8-AG in the buffer solution of HAc-NaAc (pH3.60) there is a distinct oxidation peak, the peak potential of about 1.20V (vs SCE), double-stranded DNA molecules, both of occurred between the role of electrostatic attraction, 8-AG oxidation peak clearly positive shift; same time as the increase of the amount of reaction time, temperature, and DNA, both more intense role. Both after 90min ,8-AG no longer damage the DNA of normal cells. The study determined the corresponding kinetic parameters, to create a method for quantitative detection of DNA concentration. Also on the role of the 8-AG with a double-stranded DNA (dsDNA) and single-stranded DNA (ssDNA) to generate the response to the type and sensitivity of the assay with bare electrodes in DNA modification were compared. 4: 6 - mercaptopurine (6-MP) on the hanging mercury drop electrode voltammetric behavior using a variety of electrochemical method. In phosphate buffer solution (pH7.40) in, 6-MP on the hanging mercury drop electrode controlled by adsorption of redox peaks, the reduction peak oxidation peak current and the concentration of 6-MP in 9.00 × 10 -6 mol / l ~ 1.00 × 10 -4 mol / L and 4.00 × 10 -5 to 1.00 × 10 -4 mol / L range has a good linear relationship between the detection limit of 3.00 × 10 -7 mol / L and 1.00 × 10 -7 Tmol / L, respectively. Thereby establishing a 6-MP electrochemical methods and the determination of the method for the determination of the content of 6-MP in the Lok disease rather tablets quantitative analysis and recovery with satisfactory results.

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CLC: > Mathematical sciences and chemical > Chemistry > Analytical Chemistry > Instrument analysis ( physics and physical chemistry ) > Electrochemical analysis
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