Dissertation > Excellent graduate degree dissertation topics show

Electrochemical Study of Interaction between Anticancer Drugs and DNA

Author: DongYanMin
Tutor: ZhouChangLi
School: Jinan University
Course: Chemical Engineering and Technology , Analytical Chemistry
Keywords: Anti-cancer drugs DNA Electrochemical methods Interaction
CLC: R96
Type: Master's thesis
Year: 2010
Downloads: 69
Quote: 0
Read: Download Dissertation

Abstract


This article electrochemical method the anticancer drugs Magnolol, methotrexate, aminoglutethimide, leucovorin electrochemical behavior of its interaction with biological macromolecules DNA. The use of layers of self-assembly, the potential deposition technology preparation of the modified electrode was investigated media type and concentration, pH, scanning speed of the electrochemical behavior of anti-cancer drugs. By cyclic voltammetry, linear scanning, differential pulse method, timing electricity and other electrochemical techniques to obtain the kinetic parameters of anti-cancer drugs. Way of interaction of anti-cancer drugs and DNA, the calculated binding constant and the number of binding sites. And verified by spectrometry. Research glassy carbon electrode electrochemical behavior of Magnolol in 0.1 M PBS (pH 7.0) solution Magnolol the 0.449V at oxidation peak, the electrochemical oxidation of an electron and a proton is completely irreversible The process of electron transfer coefficient (alpha) was 0.441 ± 0.001. The honokiol 100-450 mV / s scan speed range of the electrochemical reaction at the electrode surface by surface control electrode reaction is a diffusion controlled process at high scan rate of 600-950 mV / s, the corresponding electrochemical rate constant (ks) 0.0760 ± 0.0001 s -1 . The diffusion coefficient (D) and the surface of the adsorbed amount (Γ) is (3.76 ± 0.01) × 10 -7 cm 2 / s, and (2.98 ± 0.01) x 10 < sup> -10 mol / cm 2 . Electrochemical methods and spectroscopy study Magnolol and DNA interactions, Magnolol DNA in the manner combined binding constant and the number of binding sites were 1.14 × 10 5 M - 1 and 0.973. By optimizing the conditions established honokiol the method of measuring the linear range was 4.50 × 10 -8 -1.70 × 10 -7 M and 3.00 × 10 - 6 M-1.80 × 10 -5 M, limit of detection (S / N = 3) of 1.50 × 10 -9 M. 2 layers prepared by electrostatic self-assembly (DNA / CS) 6 / PGE electrode as an electrochemical probe of methylene blue modified electrode using cyclic voltammetry and electrochemical impedance method characterized. Methotrexate electrochemical behavior studies have shown that, in the range of 40-200mV / s scan rate, the electrode reaction is a surface-controlled process, while increasing the scanning speed, methotrexate for the diffusion-controlled electrochemical reaction on the electrode surface process. Proton transfer and the ratio of the number of electrons transferred for the m / n = 0.483. Methotrexate interaction with DNA studies show that methotrexate and DNA molecules in insert mode, combined with constant and number of binding sites were 6.31 × 10 5 M -1 and 1.86. The results obtained by UV spectroscopy and fluorescence spectroscopy and electrochemical methods. Determination of methotrexate, and its detection limit is 5.0 × 10 -9 M. Prepared mesoporous materials SBA15 modified carbon paste electrode, cyclic voltammetry modified electrodes were characterized. The aminoglutethimide electrochemical behavior of studies have shown that aminoglutethimide a pair of redox peaks at 0.791V, 0.078V and 0.257V at a separate oxidation peak. Aminoglutethimide electrochemical behavior of a multimodal irreversible process. Within the scan range of 20 to 150 mV / s, aminoglutethimide electrochemical reaction at the electrode surface is a surface control was within the range of 200-600 mV / s scan rate, the electrode reaction is a diffusion controlled process, the corresponding electrical chemical rate constant of 2.41 ± 0.01 s -1 , the electrode surface diffusion coefficient D for the 2.12 × 10 -3 cm 2 / s, surface concentration (5.87 ± 0.01) × 10 -11 mol / cm 2 . Aminoglutethimide and DNA interaction studies have shown that aminoglutethimide with DNA insert mode, the binding constant and the number of binding sites for β = 2.41 × 10 5 M -1 and m = 1.69. By optimizing the conditions established the aminoglutethimide method of measuring the linear range was 4.30 × 10 -7 M-8.60 × 10 -6 M and 9.60 × 10 -6 M-1.08 × 10 -4 M, the limit of detection (S / N = 3) to 4.62 × 10 -9 M. (4) prepared using electrodeposition method of Nafion / Au / GCE electrode modified electrodes were characterized by cyclic voltammetry. The electrochemical behavior of leucovorin, leucovorin, an oxidation peak at 0.570V, the electrochemical oxidation is a completely irreversible process, the number of electron transfer and proton transfer ratio of n: m = 2:3. Low scanning speed range of 20-180 mV / s, leucovorin electrochemical reaction by the apparent control of the electrochemical rate constant ks = 0.516 ± 0.001 the s -1 . In the range of 200-600 mV / s scan speed, the electrochemical reaction is diffusion controlled. Leucovorin, in Nafion / Au / GCE surface of the diffusion coefficient and the electrode surface adsorption capacity (2.44 ± 0.01) × 10 -5 cm 2 / s (3.03 ± 0.01) × 10 -10 mol / cm 2 . The leucovorin, DNA Interactions, leucovorin, and DNA by electrostatic force, combined with the constant beta and the number of binding sites m M-1 and 1.83 to 4.89 × 10 5. Establish a method for determination of leucovorin, optimum conditions, the linear range is 6.42 × 10 -6 M-1.18 × 10 -5 M, the detection limit to 2.04 × 10 < sup> -8 M.

Related Dissertations

  1. Research on Combinatorial Regulation of Multiple Transcription Factors,Q78
  2. Study of Interaction between Epoxy Molecules and Carbon Fiber Surface with Molecular Simulation,TB332
  3. The Research on Interaction in the Public Crisis of Government and the News Media in China,D630
  4. Effects of Light, Temperature and Nitrogen on the Growth of Three Kinds of Aquatic Plants,Q945
  5. Growth Rate and Growth Model of the F5 Selected Lines for Four Shell Colors of Pinctada Martensii （Dunker） Cultured in Deepwater Area of Beibu Bay in Zhanjiang and Their Genetic Diversity Analysis Using SSR Molecular Markers,S968.31
  6. An Action Research on the Influence of Classroom Interaction on High School Students’ English Speaking Proficiency,G633.41
  7. Research for Social Interaction of Interest-related Groups,C912
  8. Functional Analysis of Proteins Encoded by RNA2 of Wheat Yellow Mosaic Virus,S435.121
  9. Treatment Efficiency of Entecavir and TACE in Patients with Primary Heptatic Carcinoma of the HBV Copying,R735.7
  10. Establishment of the Molecular Identifying System of Curvualria and Application in Difficult Species,Q949.32
  11. Research on Structural Vibration of Steam Piping System,TK284.1
  12. Research on Construction Accident Factors’ Interaction Relations,TU714
  13. Detection of Exogenous Recombinan DNA of Transgenic Rice and Its Rice Product,S511
  14. Yeast Two-Hybrid Screening of 14-3-3-Interacted Proteins During Early Cotton Fiber Development,S562
  15. On Governance Optimization in the Vision of the Network Politic,D630
  16. Research on Teaching Interaction of Collaborative Knowledge Building in Web Environment,G434
  17. A Study of Theory and Practice in Interatcion between Primary Schools and Community Areas,G621
  18. Research of the Partnerships Construction of Schools and Communities,G637
  19. Hydrophilic structure of different types of surface -modified polyurethane material and coagulation 12 factor nine peptide fragments,and fibrinogen P1 fragment of computer simulation of the interaction,O631.3
  20. Primary Studies on Transcriptome and Population Genetics of An Invasive Weed Mikania Micrantha,S451
  21. Investigation of Cereal Cyst Nematode on Wheat in Jiangsu Province and the Sequence Analysis of rDNA-Its Region from Jiangsu Populations,S435.121

CLC: > Medicine, health > Pharmacy > Pharmacology
© 2012 www.DissertationTopic.Net  Mobile