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Growth inhibition of calcium oxalate crystals

Author: YueWenZuo
Tutor: ShenYuHua
School: Anhui University
Course: Inorganic Chemistry
Keywords: Calcium oxalate Urinary stones Biomineralization Crystal growth Soft template
CLC: O782
Type: Master's thesis
Year: 2004
Downloads: 289
Quote: 5
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Abstract

As we all know, homogeneous nucleation in the crystallization process of nuclear and non-homogeneous nucleation are two possibilities, the use of organic matrix template, inducing the growth of inorganic crystals, simulated in vivo mineralization process actually promote heterogeneous nucleation inhibition of homogeneous nucleation. Urinary stones abnormal biomineralization product composed of inorganic crystals and organic matrix. Which is the main component of the crystal is calcium oxalate, there are three main types: calcium oxalate monohydrate (COM, monoclinic system), calcium oxalate dihydrate (COD tetragonal crystal system) trihydrate (COT, triclinic lines), which COM strongest and tubular epidermal cell adhesion, is the main component of calcium oxalate urinary stones. Thermodynamically unstable COT played a pioneering role in the process of the formation of calcium oxalate stones. Urinary stones matrix including proteins and polysaccharides, they play an important role in the formation of urinary stones. Biomineralization is a complex process, it is difficult to be simulated in vivo and in situ, using in vitro simulation to explore the mechanism of formation of urinary stones, is important not only for the prevention of urinary stones, and more non-invasive treatment of urinary stones opened up a road. We choose monolayers, microemulsions, biological molecules as of template induced calcium oxalate crystal growth, and to explore the promotion and inhibition of calcium oxalate crystal growth factor. Supersaturated solution containing different concentrations of human serum albumin (HSA) by comparing calcium oxalate subphase pressure on mixed monolayers of stearic acid / octadecylamine (OA / OAM) - single-molecular area (π-A) isotherms, differential curve and OA / LB films of the OAM mixing UV-vis spectra, FT-IR spectra and fluorescence emission spectra, can be seen, HSA adsorbed on the OA / OAM film, so that the OA / OAM film properties changed, and the impact to the OA / OAM monolayers induced crystallization of calcium oxalate role. When subphase HSA concentration increased from 0.00% to 0.10%, Monolayer different morphologies of calcium oxalate crystals were COT, COD and COM. With the increase of the HSA concentration, COT the crystals gradually increases, indicating that the HSA can promote COT crystal growth. When the sub-phase solution of HSA concentration to a specific value generated COM crystals. Dynamic observation on the COM the formation process found through the process of transforming state crystal → COM from COT → transition, the whole process takes about 480 hours. The use of the present composition into 1,2 - bis (o-amino-phenoxy) ethane-N, N-N 'N'-tetraacetic acid (BAPTA), we successfully suppress this transformation ,2-BAPTA further study, it is expected to play a role in the prevention and suppression of urinary stones. To select the polyethylene glycol octylphenyl ether (OP) / 2 - ethyl hexanol (isooctanol IOAs) / cyclohexane / water micro-emulsion is formed of the analog system, the use of a surfactant as a template induced CAC < sub> 2 O ₄ crystal growth, the hair Anhui University to apply for a master's degree thesis now caCZo; mainly along caz enriched the coM the (01)-oriented growth. To this microemulsion was added to a certain concentration of amino acids (tryptophan, tyrosine, aspartic acid), calcium oxalate crystal growth primarily amino acid induction. Amino acid surface carried by the electrical properties of the charge to have a major impact on crystal growth. When its surface with a positive charge, can promote CaCZO; along the COM (020) plane growth. When its surface with a negative charge, can promote the COM (i0l) surface growth and CaCZo; degree of growth along the plane orientation with tryptophan tyrosine ^{days aspartate enhanced. The above experiments show that the body of tryptophan, tyrosine, and in particular is aspartic acid or contains these amino acids of the protein can promote the formation of urinary calculi. Human urine contains uric acid, citric acid and other biological molecules as templates induced calcium oxalate crystal growth, and growth is compared with the pure water of calcium oxalate crystals. The results show that the pure water system is formed along a plurality of crystal growth, a variety of polymorphs of calcium oxalate hydrate, uric acid template COT generation is suppressed, but obviously promote the (020) plane of the COM and COD (1 00) the growth of the surface, and the mixture of citric acid and uric acid template inhibited the growth of COT, COD and COM along the (020) surface growth, so CaCZO4 almost all along the COM (i01) face growth. This mixed template selectively inhibit or promote the growth of different types of calcium oxalate crystals, but the overall result was to play a catalytic role-based on urinary stone formation.}

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