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Purpose from the nerve functional morphology, biochemistry and gene expression were observed of wisdom particles of β-amyloid (β-Amyloid protein, Aβ) induced by the intervention of the role of the degeneration of nerve cells in the brain, as well as with apoptosis, apoptosis-related genes expression of the relationship, the to explore the of wisdom particles to treat Alzheimer's disease the mechanism of action. The rat basal nuclear micro-injection of Aβ modeling, divided into the model group, of wisdom particles high dose group, middle dose group and low dose group, nimodipine positive control group; Moreover, a normal control group, the basal ganglia microinjection of physiological salt water. 30 rats in each group were fed with corresponding drugs (model group, the control group was given NS), day 1. Y-maze test, respectively 15, 30 days, testing the ability of learning and memory in rats. 30 days after the administration, the rats were anesthetized to 4% paraformaldehyde perfusion hearts take hippocampus preparation of paraffin sections, HE staining, of TUNEL cells dyeing. In another fresh hippocampal tissue the electron microscopy apoptosis; extracted DNA electrophoresis on agarose gel; immunohistochemical assay of Bax, Bcl-2 expression in the hippocampus, semi-quantitative image analysis scoring. Results of the behavioral indicators show that the The model rats increase in the number of attempts, of learning and memory impairment, and comparison with the normal control group, a significant difference (P <0.01). The raw the Hui particle group, nimodipine group can significantly improve the learning and memory ability of AD rats, compared with model group was significantly different (only 0.01). HE staining showed that the model of hippocampal cell degeneration of wisdom particles nimodipine group was significantly reduced (only 0.01). TUNEL staining, DNA electrophoresis and electron microscopy showed that the model group, the typical features of apoptosis, and of wisdom particle group, the the nimodipine group can be reduced apoptosis. Than the normal control group, model group, the expression of Bax was significantly increased (only 0.01), BCI-2 expression was significantly reduced (only 0.01); Sang Hui particle group and the nimodipine group hippocampus Bax expression level compared with the model group decreased significantly (only 0.01 ), the expression of Bcl-2 had no significant change (corpse gt; 0.05); Bax / BCI were significantly reduced in the hippocampus of wisdom particles group (only 0.01). Conclusion p-amyloid protein in the basal ganglia microinjection can cause learning and memory impairment in rats; cause degeneration of hippocampal cells, typical apoptotic phenomenon upregulation of apoptosis-related genes bax, bcl-2 downregulation, bax / bcl 2 ratio increase. Of wisdom particles significantly improve learning and memory impairment in rats induced by Ap; alleviate Ap damage to hippocampal cells, inhibition of apoptosis, and regulation of apoptosis related genes Bax, Bd-2 expression.
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