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Screening the Antimicrobial Activities of Microorganism for Food Preservative and Study on the Active Substance for Them

Author: WangYan
Tutor: YangZiWen
School: Wuhan Institute of Virology
Course: Microbiology
Keywords: Active substance Extract Bacillus licheniformis Orthogonal test The inhibitory spectrum
CLC: TS20
Type: Master's thesis
Year: 2007
Downloads: 386
Quote: 0
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Abstract


Mixed bacteria , Salmonella typhimurium , Escherichia coli , Staphylococcus aureus , yeast , Bacillus subtilis and Bacillus subtilis as the the corruption indicator bacteria from soil samples , samples of organic matter , various pickles and traditional food , large fungi , all kinds of seafood , plant samples were separated . Active strains were isolated 600 strains of higher activity initial classification : Bacillus licheniformis , Bacillus cereus , Bacillus polymyxa . 1801 were made by the representatives of active substances extracted . Fermentation in TYG liquid medium 48h, 6000 r ยท min -1 centrifugal 20min , the centrifugal supernatant PH3 precipitation and extracted with 80% ethanol extract pumping crude extract . Crude ethanol extract can be completely dissolved in alkaline water . The antibacterial substance stability test : crude extract of proteinase K, heat , pH stable . Explore : the separation and purification of the crude extract made ??after preparation and secondary preparation and purification by HPLC analysis by LC-MS analysis ; too macroporous resin ; through the ion exchange column by LC - MSMS analysis . LC-MS spectra , which contains 1452,1466,1480,1494,1508 and 1030,1044,1058 two groups homologues . This paper reports the antagonistic bacteria separation , identification, evaluation of antibacterial effect and the active substance preliminary purification and physical and chemical properties . Medium optimization test of Bacillus licheniformis 1801 . 1801 main influencing factors pH , inoculum size , the amount of sample loading single factor test to determine the optimum pH was 7.2 , inoculum size of 5%, the amount of sample loading 100 ml . Tryptone , glucose, yeast for three factors and three levels orthogonal test . To obtain the optimization of the medium composition : Final draw optimize the medium tryptone 1 % , yeast extract 0.02% , glucose 0.5 % , the NaCl as 0.5 % , pH7.4 . Micrococcus luteus (Micrococcus luteus) bacteria indicator bacteria screening , screening a total of 3930 actinomycetes . Double flat rescreening , The race elect 14 active strains . Which selected strains of active , fast growing , short period of five active strains , antibacterial spectrum detection . Identify high activity , fast growing , short cycle , antibacterial broad spectrum of strains 2925 , and the classification and identification , identified in 2925 as a small sp .

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