Dissertation > Excellent graduate degree dissertation topics show

Isolation and Structure Elucidation of Chemical Composition from the Fruit of Cucurbita Moschata Duch

Author: WangZuoJie
Tutor: DuZuoZhen
School: Zhejiang Technology and Business University
Course: Of Food Science
Keywords: Cucurbita moschata Duch. Chemical constituents Separation Structure elucidation
CLC: TS255.1
Type: Master's thesis
Year: 2007
Downloads: 369
Quote: 2
Read: Download Dissertation


Pumpkin is the fruit of Cucurbita moschata Duch which belongs to cucurbiaceae family. It has been used in food and medicine for thousands of years in China. The total output of pumpkin is 1.8 million tons in China, which is about 30% of world total output. This vegetable is very popular in China because it is thought to possess pharmaceutical effect such as lowering sugar and blood pressure. But there is little knowledge about the bioactive chemical constituents besides polysaccharides in pumpkin. In this study we focus on the chemical constituents of pumpkin.1. The chemical constituent in the chloroform extract in pumpkins was separated with silica gel column chromatography to yield 4 compounds: Cis-15-vaccenic acid (Ⅰ), Steraric acid (Ⅱ), Cis-15-vaccenic acid methyl ester (Ⅲ) and Sugar (Ⅳ), elucidated by MS and NMR data. These compounds were isolated from pumpkin for the first time. Cis-15-vaccenic (Ⅰ)Steraric acid (Ⅱ)Cis-15-vaccenic acid methyl ester(Ⅲ)Sugar (Ⅳ)2. The n-butanol extract of pumpkins was isolated by D0402 macroporous adsorption resin eluted with water, 20%, 40%, 60%, 80% and 95% ethanol to obtain the components with different polarity. The fraction of 60% ethanol was isolated by HSCCC using a two-phase solvent system composed of light petroleum-chloroform-methanol-n-propanol-water(1: 5: 6: 1: 4, v/v) as the solvent system to yield three components BU1, BU2 and BU3. The component BU1 was isolated by HSCCC using a two-phase solvent system composed of light petroleum-chloroform-methanol-n-propanol and water (3: 5: 6: 1: 4, v/v) to yield a component BU1-2. The components BU1-2, BU2 and BU3 were isolated by preparative HPLC to yield compoundsⅤ-Ⅸrespectively. CompoundsⅥ-Ⅺwere elucidated as moschglycolipid A-F, on the basis of ESI-MS, ESI-MS-MS and NMR analysis. These compounds may be new based on our searches in several data bases.Moschglycolipid A(Ⅵ)Moschglycolipid B(Ⅶ) Moschglycolipid C (Ⅷ)Moschglycolipid D (Ⅸ)Moschglycolipid E (Ⅹ) Moschglycolipid F (Ⅺ)

Related Dissertations

  1. Numerical Investigation of Effects of Boundary Layer Suction on the Performance of Diffusion Cascade,TH453
  2. The Flow Theroy Investigation of Compressor Cascade with Split,TK474.8
  3. The Chemical Constituents of Bryozoan Bugula Neritina L. and Junceella Fragilis from South China Sea,R284
  4. Synthesis and Resolution Property of N-(9-Fluorenylmethoxycarbonyl)-D-Tryptophan and D-Phenylalanine Imprinted Polymer,O631.3
  5. Experimental Study and Analyses of O2 -CO2 Production Based on Chemical Looping Method,O611.3
  6. Synthesis and Seperation of 1,1’-binaphthol,O625.3
  7. Screening of Plant Pathogen Inhibition of Plant Extracts,S482.2
  8. Identification of Marine Sreptomyces Sp. GY-4 and Researches on Its Antimicrobial Substances,Q936
  9. Prokaryotic Expression and Purification of Human Beta-Defensin-9,Q78
  10. Study on Separation、 Preconcentration and Analysis of Trace Elements with Carbon Nanometer Materials,TB383.1
  11. The Research on the Microwave Stability of Selenium-Enriched Rich Bram,TS210.9
  12. Isolationand Identification of Mixed Photosynthetic Microbial and Their Hydrogen Production,TQ116.2
  13. Chemical Studies on the Roots and Rhizomes of Helleborus Thibetanus,R284
  14. Preparation of Polysulfonate Ultrafiltration Membrane and Application on Oil Decolorization,TQ320.721
  15. Preparation, Separation of Monascus Pigments and Structural Characterization of Monascus Yellow Pigment,TS264.4
  16. Studies on the Extraction, Saparation and Purification of Red Pigment and Procyanidin of Red Skin of Peanuts,TS202.3
  17. Study on the Characteristics and Simulation of the Hydrocyclone Reparation Process for Initial Rainwater,X522
  18. To Study the Techniques for Purification of Nature Products by High-speed Counter-current Chromatography(Ⅱ),R284
  19. Phosphate modification of nano- TiO 2 Photoelectrochemical Properties of the,TB383.1
  20. Preparation and Physicochemical Properties of ACE-inhibitory Peptides from Silkworm Pupae Protein,S886.9
  21. Purification and Identification of an Acidic Polysaccharide Fraction GFLA-1 from the Flowers of Panax Ginseng C. A. Meyer,S567.51

CLC: > Industrial Technology > Light industry,handicrafts > Food Industry > Fruits, vegetables,nuts processing industry > Basic science
© 2012 www.DissertationTopic.Net  Mobile