Dissertation > Excellent graduate degree dissertation topics show

Development and Characterization of Triticum Aestivum-Hordeum Californicum Alien Chromosome Lines and Powdery Mildew Resistance Evaluation

Author: KongFang
Tutor: WangXiuE
School: Nanjing Agricultural College
Course: Crop Genetics and Breeding
Keywords: T. aestivum H.californicum alien addition lines chromosome C-banding/fluorescence in situ hybridization (FISH) Karyotype SDS-PAGE
CLC: S512.1
Type: Master's thesis
Year: 2006
Downloads: 81
Quote: 0
Read: Download Dissertation

Abstract


Sequential chromosome C-banding/Fluorescence in situ hybridization （FISH） wasconducted on Root-tip cell （RTC） chromosomes of H. californicum and Triticum aestivumc.v. Chinese Spring-H. californicum amphiploid. The results showed that mostchromosomes of H. caIifornicum had strong terminal bands. Different chromosomes couldbe distinguished by their unique band pattern and could be identified in the wheatbackground. The FISH results using labeled 45SrDNA as probe revealed that there weretwo hybridized signals on a pair of H7 chromosomes of H. californicum. Based on thechromosome C-banding and FISH results, karyotype analysis was conducted using Moticimages plus 2.0ML software. The karyotype formula of H. catifornicum was 2n=2x=10m（2SAT）+4sm.T. aestivum L. cv. Chinese spring （CS）-H.caIifornicum amphiploid was crossed to CS,then backcrossed and self-fertilized. Among the BC2F4 progenies, the genetic constitutionof backcross progenies were analyzed by morphology observation, sequentialC-banding/FISH on root tip cell chromosomes, configuration analysis of pollen mother cell（PMC） at MI, biochemical and molecular marker analysis. Three disomic addition（DA2H;DAH1; DAH4）, three monosomic addition（MA5H; MAH1; MAH4）, two telosomic addition（DtH4, MtH4） and one multiple addition lines were identified. Among them, all theH.californicum chromosomes except H5 were involved. STS-PCR and RFLP（Restrictedfragment length polymorphism） analysis using 2BL specific markers indicated that H3chromosome belongs to the wheat homoeologous group 2, and hence could be designatedas 2H. SDS-PAGE showed that H2 chromosome belongs to the wheat homoeologous group5, and hence could be designated as 5H. All the parent lines and the identified alienchromosome lines were evaluated for powdery mildew resistance. The CS-H.californicumamphiploid showed better powdery mildew resistance than CS. Addition lines DAH1,MAH1, DAH4, MAn4 and the multiple addition lines also showed good powdery mildewresistance, indicating that chromosomes H1 and H4 might involve powdery mildew resistance of H.caIifronicum.The identified powdery mildew resistant addition lines shouldhave great potential value in wheat breeding for powdery mildew resistance.

Related Dissertations

  1. Isolation, Purification and Antioxidant Activity of Proteins from Chinese Yam,TS255.1
  2. Molecular Cytogenetics Analysis of Backcrossing Progenies of the Irradiated Triticum Aestivum-Hordeum Californicum Amphiploid,S512.1
  3. Development and Identification of a Triticum Aestivum-Thinopyrum Bessarabicum Chromosome Translocation Line and Analysis on Its Genetic Effect,S512.1
  4. Seed Proteomic Analysis of TrxS Transferred Barley Lines,S512.31
  5. Chinese freshwater planarian classification and karyotype studies (Ⅺ),X174
  6. The Improvement of Quantitative Detection of γ-PGA by Hydrolysis and the Research of Biosynthetic γ-PGA’s Molecular Weight Distribution and YWTD,TQ920.1
  7. The Significance of Quantitative Analysis of Dysplasia in the Diagnosis and Prognosis of MDS,R551.3
  8. Guangxi flat orange and some local citrus germplasm resources survey and analysis of genetic relationship,S666
  9. Biologica, Clinical and Hematologic Characteristics of a Rare Acture Myeloegakaryoctic Leukemia,R733.7
  10. Study on Proteinase Which Microbe Secreted from Traditional Fermented Soybean (Douchi) in Yunnan Province,TQ925
  11. Experimental Study on the Antimutagenic Effects of Prepared Radix Rehmanniae Polysaccharides,R285.5
  12. Studies on Photosynthetic Characteristics, Cytology and Content of Total Alkaloids of the Genus Lycoris from Anhui Province,S682.29
  13. Determination the Activity of Alliinase in Fresh Garlic and Study the Stability of the Alliinase and Alliin/Alliinae Enteric Capsule,R283
  14. Isolation of Adherence-Defective Mutants and Research of Related Cellulose Binding Membrane Proteins,Q933
  15. Preliminary Proteomics Analysis of Lactobacillus Plantarum,Q946
  16. Composition Analysis of Glutenin Subunits in Shaanxi Wheat and Establishment of Multiplex PCR System for Dough Strength Based on Molecular Markers,S512.1
  17. Molecular Genetics of Somatic Hybrids between B. Oleracea and B. Nigra,S565.4
  18. Development and Identification of Hybrid Offsprings between Wheat and Thinopyrum Intermedium,S512.1
  19. Identification and Evaluation of Proteins Related to Renal Acute Allograft Rejection,R699
  20. Purification and Its Clinical Application of Humanized S-Adenosyl-L-Homocysteine Hydrolase,R346
  21. Establishment of Two-dimensional Electrophoresis System for Comparative Proteomic Analysis in Different Parts of Panax Quinquefollium L.,R284

CLC: > Agricultural Sciences > Crop > Cereal crops > Wheat > Wheat
© 2012 www.DissertationTopic.Net  Mobile