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Study on Antioxidative Components of Iris.halophila Pall.Root

Author: LiXiaoFei
Tutor: LiGuan
School: Xinjiang University
Course: Biochemistry and Molecular Biology
Keywords: I.halophila Pall. anti-oxidative activity DPPH means iso-flavones
CLC: R284
Type: Master's thesis
Year: 2007
Downloads: 103
Quote: 1
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Abstract


The relations of oxidation and disease, food rot were reviewed, researches on natural anti-oxidative products and chemical compounds from Iris L.and Iris.halophila Pall. were also summarized in this thesis.The predictive-analysis test showed that the main components in 95% ethanol extract are flavones、phenolic compounds、amylose、organic acid。The 95% ethanol extract of I.halophila Pall. root was divided into ethyl-acetate fraction, n-butanol fraction, aqueous fraction and petroleum-ether fraction, the anti-oxidative activities of different fractions except petroleum-ether fraction were analyzed by DPPH means and estimated by EC50 and AE parameters which were compared with BHT’s, UV absorption spectrum was also used to analyze the main components in these fractions. As a result, three fractions all have DPPH radical scavenging activity, the order of EC50 is BHT>n-butanol fraction>aqueous fraction> ethyl-acetate fraction, meanwhile the order of AE is n-butanol fraction >BHT> aqueous fraction > ethyl-acetate fraction, it was indicated that n-butanol fraction has better antiradical efficiencies(AE) than BHT’s, the UV absorption spectrum indicated that the main components in every fraction are iso-flavones.Four compounds were isolated from ethyl-acetate fraction by using chromatography technology, and they are first isolated from I.halophila Pall., the structures of the four compounds were identified by using UV、IR、MS、NMR and X-ray diffraction technologies. The four compounds are 6,4’-dimethoxy-5,7-dihydr-oxy-isoflavone;5,7,3’-trihydroxy-6,7,4’-trimethoxy-isoflavone(Irigenin);5,4’dihydroxy-6,7–methylenedioxy-isoflavone(Irilone) and 5-Methoxy-4’-hydroxy-6,7-methy-lenedioxy-iso-flavone(Nigrigin).Nigri- gin was characteri-zed by single-crystal X-ray diffraction, bond lengths and bond angle of Nigrigin molecular were studied by X-ray diffraction. The detail document of Nigrigin molecular was registered in Cambridge Crystallograp- hic Data Centre(CCDC), the registered number is: 645846.Anti-oxidative activity of the four compounds were analyzed by DPPH means, the result is that the activity of 5,4’-dihydroxy-6,7-methyllenedioxy- isoflavone is better than other three compounds, but lower than BHT’s.

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