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Objective: To investigate the proliferative capacity of different cells exists in rat liver cancer, and preliminary study on the biological characteristics of high proliferative capacity cell subsets, compare differences with liver cancer cell susceptibility characteristics. Methods: 1, limited dilution monoclonal cultured rat hepatoma cells, to identify different proliferation of liver cancer cell subsets (HSG 1, HSG-2), and the characteristics of cell growth, cell cycle distribution, tumorigenicity and sustain the culture under differentiation capacity and other biological characteristics. 2, take HSG-2 liver cancer cells, doxorubicin (0.2μg/mL), fluorouracil (5μg/mL) and cisplatin (1μg/mL), the role of 72h, SRB method were used to detect chemotherapy drugs HSG-2 with HCC cell inhibition rate. Results: (1) a total of 6 rats cultured relatively stable continuous proliferation subsets, wherein the stabilizer appears two subsets were named as HSG-1 with the HSG-2; (2), the HSG-2 cell doubling time, the largest the hyperplasia multiples, cell cycle distribution were significant differences. (3) the HSG-2 cells into tumor proportion was 100% after logarithmic 1/10 dilution, in the proportion of 50% in the tumor, and the inoculation of the original density of the corresponding subsets 0 compare, P = 0.001; (P = 0.023), were There are significant differences. (4) HSG-2 cells subcloned culture, produced offspring clone colony morphological heterogeneity of cells the same to produce spindle and polygonal cells; polygonal clone colony turn planting to 24-well plates expanding culture can be seen Ibid two different forms of the colony, HSG-1 cells subcloned culture failed to produce polygonal daughter cells. HSG-2 subclones of offspring polygonal cells planted subcutaneously into nude mice, tumor formation rate of 100% (6/6); HSG-2 progeny spindle cells planted to nude mice no tumor. (5) doxorubicin, fluorouracil and cisplatin liver cancer in rats HSG-2 inhibition rate with inhibition rate of liver cancer cells have more significant difference, t values ??and P values ??were: t = 4.321, P = 0.002; t = 4.965, P = 0.001 and t = 4.285, P = 0.002. (6) doxorubicin, fluorouracil, cisplatin IC50 of liver cancer in rats HSG-2 with IC50 hepatoma cells have more significant difference, t values ??and P values ??were: t = 10.604, P <0.001; t = 5.642 , P <0.001 and t = 9.470, P <0.001. Conclusion: HSG-2 cell proliferation, tumorigenic ability, there is some differentiation capacity, with the initial characteristics of the stem cells of the rat hepatoma. Chemotherapy drug for liver cancer in rats HSG-2 inhibition is less than inhibition of liver cancer cells, prompt the cancer stem cells in the liver cancer and other solid tumors TSC, a unique mechanism of resistance.
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