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The rapid development in recent years, based on silica putamen nanoparticles, these nanoparticles are chosen most of the nuclear material for the of - (bipyridine) ruthenium (Rubpy) magnetic materials, fluorescein, rhodamine, rare earth elements with the and methylene blue dye, and has been in many areas of biological analysis of a wide range of applications. The while the putamen type of nano-particles of cyanine dyes study is rarely reported, the thesis preparation and properties of silica coated phthalocyanine dye the putamen type nanoparticles. Reverse microemulsion method four kinds of water-soluble cyanine dyes, Rubpy, methylene blue and Cy2 dye phenomenon differences in the preparation of nanoparticles, the Rubpy, methylene blue and Cy2 dye nanoparticles under TEM , spectroscopic properties, properties such as the dyes washed leakage performance. Combined to form the reaction process of the nano-particles obtained cyanine dyes capable of forming a stable core-shell type nanoparticles structural features are: a water-soluble dye molecules and enriched with a positive charge. Synthesis of Cy3 and Cy5 dyes of the structure of the two molecules with three positive charges, one each, the dye molecule by mass spectroscopy, H spectra and associated with HH spectrum characterization. Cyanine dyes nanoparticles prepared by a reverse microemulsion method, uniform particle size, monodisperse, the average particle size of 50nm (Cy3) and 60nm (Cy5), to study the spectral properties of nanoparticles and dye characteristics, and explore the dye soaked in the water leakage properties, it is found that after a 48 hour soak, the nanoparticles were still holding 82% (Cy3) and 64% (Cy5) fluorescence intensity. Cy3 dye and light stability properties of the nano-particles, and after 90 minutes of illumination, the intensity of the dye fluorescence to 15%, the nanoparticles still maintaining 78% of the fluorescence intensity. The synthesized molecular structure introduces APS (aminopropyl triethoxysilane) a Cy7 dye, and by MS, H-spectrum and associated with HH spectral characterization. Cy7 Stokes shift of nearly 140nm covalently coated with it prepared putamen type cyanine dye nanoparticles monodisperse, with an average particle size of 65nm and 120nm Stokes shift reaches. After soaking in water for 72 hours, the fluorescence intensity of the nanoparticles is maintained at more than 96%, the intensity of the dye fluorescence to 33% after 20min the continuous irradiation, and the the nanoparticle fluorescence intensity remained at 53%, to a certain extent improve the light stability sex.
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