Dissertation > Excellent graduate degree dissertation topics show

Cloning and Sequence Analysis of Complete Genomic Sequence from the Inner Mongolia Strain of Endogenous Jaagsiekte Sheep Retrovirus

Author: WangYu
Tutor: HanMin;LiuShuYing
School: Inner Mongolia Agricultural University
Course: Clinical Veterinary Medicine
Keywords: Ovine pulmonary adenocarcinoma(OPA) Jaagsiekte sheep retrovirus(JSRV) Endogenous Jaagsiekte sheep retrovirus(enJSRV) Dot blot hybridization Clone Sequence analysis Structure prediction
CLC: S852.65
Type: Master's thesis
Year: 2007
Downloads: 67
Quote: 1
Read: Download Dissertation

Abstract


In order to amplify the complete genome of enJSRV from the strain of inner Mongolia (enJSRV-NM),by using enJSRV-specific and exJSRV-specific DNA probes in dot blot hybridization. The total DNA of enJSRV was extracted from uterus tissue of healthy domestic sheep by Clinical and pathologic examination. Seven pairs of primers were designed based on Genbank sequences. Seven fragments were obtained by PCR and were cloned into the PMD19-T vectors. The recombinant plasmids were sequenced and the complete sequences were analyzed with DNAstar software. The results showed that the genome was 7942bp in length and contained four overlapping open reading frames of the gag, pro, pol and env genes as well as an additional open reading frame (orf-x) that overlaps 3’ end of the pol gene. The nucleotide acid sequences of enJSRV-NM loci were compared with the sequences of South Africa enJS56A1 strain and USA JSRV21 strain. The nucleotide acid identities were 99.2%、92.3%, respectively. Two zinc fingers were found in the region of NC in the predicted amino acid sequence. A ScaⅠrestriction site in gag was not found in the sheep genome, the YXXM motif was not found in the region of TM, which are reliable molecular markers for the infectious exogenous virus. It was found that the enJSRV-NM are 90~98% identical at the amino acid level to their exogenous infectious counterparts in most of the retroviral genome,enJSRVs consistently differ from JSRV isolates in three variable regions (VRs). VR1 and VR2 are found in gag, whereas VR3 is at the transmembrane domain of env.This is the first nucleotide sequence of enJSRV reported in China.The protein structure prediction showed that the gag-enJSRV-NM and env-enJSRV-NM protein are a loose protein.These results pave the way for future developments on OPA.

Related Dissertations

  1. Cloning and Expression Analysis of GPx, GST and SAHH Genes in Chlamydomonas Sp. ICE-L from Antarctica,Q943.2
  2. Cloning and Characterization of BAFF Gene from Whitespotted Catshark and Crucian Crap,S917.4
  3. Survey in Nanjing and Analysis of the Mitochondrial Genome of Frankliniella Occidentalis (Pergande),S433
  4. Isolation and Identification of Low Pathogenicity Avian Influenza (H9) Virus in Henan and Study on the Biological Charactistics,S852.65
  5. Research on Influnce of Silicon and Sulfur on Arsenic Uptake and Accumulation of Rice (Oryza Sativa L.),S511
  6. Cloning Andanalysis of NAC Transcription Factor Gene in Tobacco(Nicotiana Tabacum L.),Q943.2
  7. Isolation and Identification of Infectious Bronchitis Virus and Sequence Analysis of Its S1 Gene and N Gene,S852.65
  8. Cloning 1Dx5 Gene from the Xinjiang Wheat Variety and Constructing Its Expression Vector,S512.1
  9. Evaluation on Forage Quality and Biomass Energy Characters of Inbred Vegetative Lines of Napier Grass,S543.9
  10. Oshima Ye Lu Ju CcSOS1 Cloning and Expression Analysis,S682.11
  11. The Characteristic of the Whole Hepatitis B Virus Gene, Construction of HBV Gene Eukaryotic Cell Expression Vector, and the Efficacy of Adefovir Plus Entecavir or Plus Lamivudine as Rescue Therapies in Chronic Hepatitis B Patients with Entecavir-resistant,R512.62
  12. Research on Cloning and Expression of the Bile Salt Hydrolase Gene from Lactobacillus Plantarum JPP2,Q78
  13. Identification of Resistance to Downy Mildew and Its Related Gene Cloning in Radish (Raphanus Sativus L.),S631.1
  14. The Transmembrane Fusion Protein of MnSOD Clone Expression and Function Study,Q78
  15. Functional Analysis of Plant Asparagine Synthetase Gene in Regulation of Disease Resistance,S511
  16. Based on conditional random RNA secondary structure prediction algorithm,R346
  17. Fosmid library and PCR -based screening technologies spider silk protein gene package egg and Analysis,Q78
  18. Construction of Aquaculture Water Metagenomic Library in Pearl River Delta Area and Chitinase Gene Screening and Analysis,S917
  19. Studies on the Cloning and Expression of Sterol Regulatory Element Binding Protein-1 Gene in Siganus Canaliculatus,S917.4
  20. Paralleling Genetic Annealing Algorithm with OpenMP,TP301.6
  21. Expression of Human Nerve Growth Factor in Cho Cells with Various Vectors,Q42

CLC: > Agricultural Sciences > Livestock, animal medicine,hunting,silkworm,bee > Animal Medicine ( Veterinary Medicine) > Basic Veterinary Science > Animal Microbiology ( Veterinary Microbiology, ) > Livestock Virology
© 2012 www.DissertationTopic.Net  Mobile